Pap or H-E staining

Pap test or HE staining is one of the methods for examining tumor cells. The two are described in detail: (1) Pap staining. This method is characterized by the fact that cells have pleochroic colors and are colorful and colorful. The smear staining has good transparency, clear cytoplasmic granules and clear nucleus structure. For example, the cytoplasm of squamous epithelial hyperkeratotic cells is orange; keratinocytes are pink; and cells before keratinization are light green or light blue. Color, suitable for epithelial cell staining or to observe the effect of hormone levels on epithelial cells in vaginal smears. The disadvantage of this method is that the dyeing process is more complicated. (2) Hematoxylineosin (HE) staining method This method has good staining transparency and a clear contrast between nuclear and cytoplasm. The dyeing step is simple and the effect is stable. It is suitable for sputum smear. The enamel core is purple-blue, the cytoplasm is pale reddish red, and the red blood cells are light vermilion. Basic Information Specialist classification: Oncology examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: It should be light, eat more fruits and vegetables, mix food reasonably, pay attention to adequate nutrition. Normal value The body is in a dynamic balance with no disease. Clinical significance Abnormal results: In malignant effusion, about 60% of Pap or HE staining can be found in irregular shape, uneven cell size, large nuclei and visible nucleoli, cytoplasmic darker piles or scattered distribution The malignant tumor cells are easy to see adenocarcinoma cells arranged in a glandular cavity. However, tumor cells in effusion are generally more difficult to determine the source of the tumor. The primary tumor of the thoracic cavity is mainly malignant mesothelioma, and the incidence rate is low, about 1% to 4%. Metastatic malignant tumors account for about 95%, 80% are adenocarcinoma, squamous cell carcinoma only accounts for 2% to 3%, and lymphoma or leukemia can account for 5% to 11%. People who need to be examined: Suspected to have malignant pleural effusion and other related diseases. Precautions Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Alveolar-bronchial lavage cytology was performed using a cytospin or a pellet to prepare a smear, which was subjected to HE staining, Pap staining, and microscopic examination. Includes nucleated cell counts and classifications, tumor cells, and parasite examinations. For patients with suspected malignant pleural effusion, the pleural effusion is centrifuged, and the cells in the effusion are collected by sediment smear or special cell smear centrifuge to carefully stain for tumor cells. Not suitable for the crowd Inappropriate crowd: No. Adverse reactions and risks No relevant information.