Bacteriological test

Bacteriological testing is a targeted examination of bacteria in blood or tissue. Such as sputum examination of sputum and lower respiratory secretions, bacteriological examination of blood and bone marrow specimens, bacteriological examination of urine specimens, etc. Inappropriate people: vary according to the tests performed. Basic Information Specialist classification: Infectious disease inspection and classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a state of dynamic balance and health. Clinical significance Check the infected site to determine the type of bacteria that cause the infection, and then treat the drug in a more targeted manner. Abnormal results: (1) The main symptoms of gonorrhea infection gonorrhea are frequent urination, urgency, dysuria, purulent urethra, cervix or vaginal discharge. Or gonococcal conjunctivitis, enteritis, pharyngitis, etc., or disseminated gonorrhea symptoms. (2) Cryptococcus neoformans is an important opportunity for respiratory infections. It can cause serious clinical symptoms, most commonly meningitis. (3) Diphtheria bacilli invade the body and grow only in the throat, which produces diphtheria toxin into the blood, which can cause symptoms such as myocarditis and adrenal dysfunction. (4) Symptoms of infection caused by other pathogens. People who need to be examined: patients with bacterial infections such as Neisseria gonorrhoeae, Cryptococcus neoformans, and Corynebacterium diphtheriae. Precautions Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process [Differential diagnosis] () acute purulent myelitis (acutepurulent myelitis) Staphylococcus aureus is the most common (50 ~ 60%), followed by Enterobacter and Proteus (13 ~ 18%). (2) purulent meningitis (purulent meningitis) The most common pathogens are meningococcus, pneumococci and influenza, bloodthirsty rod B, followed by Staphylococcus aureus, streptococcus, Escherichia coli, Proteus, anaerobic Bacteria, Pseudomonas aeruginosa, etc Pathogenic bacteria can be found in cerebrospinal fluid in 50% of cases. (3) Brain abscess (pyencephalus) There is no bacteria in the cerebrospinal fluid when it is not broken, and pathogenic bacteria can be found after the brain abscess is broken. (D) tuberculous meningitis (tubercular meningitis) Cerebrospinal fluid sediment or film smear acid-fast staining, as well as tuberculosis culture or animal vaccination positive. (5) ycosic meningitis (ycosic meningitis) about 60 to 80% of cases of cerebrospinal fluid smear ink staining can be found with capsular cryptococci. Cryptococcus can be isolated from cerebrospinal fluid culture and animal inoculation, but it takes a long time. (6) Primary amoebic meningitis (primaryamoebicmenigoen-cephalitis) Amoebic brain abscess (amoebicpyencephalus) Amoebic trophozoites can be found in cerebrospinal fluid. The tissue of the infected part is taken for culture, and the culture solution is taken for examination. Morphological examination classification First, do not stain specimen inspection: 1. Hanging drop method 2. Pressure drop method. Second, the stained specimen inspection: 1. Single dyeing method (Mulan staining) 2. Counterstaining method (Gram dyeing, acid-fast staining). General steps for staining specimen inspection: First, smear preparation: smear - dry - fixed. Second, dyeing: initial dyeing - mordant - bleaching - counterstaining. Not suitable for the crowd Inappropriate people: vary according to the tests performed. Adverse reactions and risks No related complications and hazards have been found.