Biochemical examination of seminal plasma

Biochemical examination of seminal plasma is a biochemical test of seminal plasma. Seminal plasma is mainly composed of accessory gonads (prostate, seminal vesicle, urethral gland) and epididymis secretions. Therefore, seminal plasma biochemical examination is important for evaluating the function of accessory gonads and studying the influence of accessory gonads on male fertility. Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: fasting Tips: Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. Normal value (1) The measurement of fructose is generally carried out by the resorcinol method, and the normal value is 256 ± 104 mg/dl. (2) Trace elements play an important role in male reproductive and reproductive endocrine functions. Zinc in normal semen is 130±56μg/ml, copper is 1.85±0.15μg/ml, and iron is 0.81±004μg/ml. (3) Citric acid was determined by furthherrmann reaction, and the normal value was 56±2.0 mg/dl. (4) Protein was measured by the double urinary method. The normal person is 3.6 ± 0.8 g / dl. (5) Acid phosphatase was measured by the β-glycerol phosphate method. The normal value is 882 ± 412 Brine units / ml. Clinical significance Abnormal results: 1. Determination of refined berry sugar content The concentration of refined berry sugar is greater than 8.3 mmol/L or 13 mmol/primary ejaculation. Reduced berry sugar is found in androgen secretion and seminal vesiculitis; sperm berry sugar is also found in congenital seminal vesicle absence, azoospermia or seminal vesicle dysplasia caused by azoospermia and retrograde ejaculation; and simple vas deferens obstructive azoospermia The fructose content is normal. 2, seminal plasma acid phosphatase determination of prostatitis, the acid phosphatase content in the seminal plasma decreased, when the acid phosphatase increased, the prostate disease (early prostate cancer or benign prostatic hypertrophy) has diagnostic value. People who need to be examined: male infertility patients. Precautions Forbidden before examination: Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. Requirements for inspection: 1. When taking semen, you can use soft soap or paraffin oil for penile massage, collect the specimens in sterile test tubes; use condoms (wash clean, no spermicides) or use semen interruption method to collect semen, but this way The amount collected is often small. 2. If the semen is not obtained by the above method, the seminal vesicle and the end of the vas deferens can be massaged through the rectum, and the urine can be collected to check whether there is sperm in the sediment. 3. Do not expose semen to overheated and cold conditions. Hand it to the doctor for reference, no more than 30 minutes. Keep warm in cold weather, and keep it in your underwear pocket when you send it. 4. If bacterial culture is to be carried out, the urethral opening should be rinsed and disinfected, and the semen should be collected in a sterile test tube. 5. If you are not giving birth, both husband and wife must go to check. Inspection process When performing biochemical examination of semen, the semen specimen should be placed in a water bath at 35 ° C ~ 37 ° C. After liquefaction, centrifuge at 1.2 ~ 1.5 ml for 15 minutes, and take the upper layer of seminal plasma for biochemical determination. Most of the tests currently carried out are fructose, trace elements, citric acid, protein, acid phosphatase, lactate dehydrogenase x and so on. (1) Determination of fructose: The resorcinol method is generally used, and the normal value is 256 ± 104 mg/dl. Fructose is mainly secreted by the seminal vesicle, which is the main source of sperm energy metabolism and is related to sperm motility. Fructose is zero, and the absence of seminal vesicles should be considered. When the seminal vesicle has inflammation, the fructose content decreases. In addition, the androgen deficiency and the fructose content in the elderly are reduced, and the amount of fructose in the seminal plasma is higher in diabetes. (2) Trace elements: play an important role in male reproductive and reproductive endocrine function. The zinc in normal semen was 130±56 μg/ml, copper: 1.85±0.15 μg/ml, and iron: 0.81±004 μg/ml. Zinc in semen can affect sperm metabolism, and is directly proportional to sperm motility and density, and can affect the gonads indirectly by affecting the pituitary gland. When zinc is insufficient, the secretion of pituitary gonadotropin is reduced, making gonadal dysplasia, or The reproductive and endocrine functions of the gonads are impaired. Copper can affect sperm survival and activity, copper concentration in semen is high, and sperm activity is poor. (3) Citric acid: measured by furthherrmann reaction, the normal value is 56±2.0mg/dl, mainly from the prostate, regulating the concentration of calcium in semen, affecting the liquefaction of semen, and having the action of prostatic acid phosphatase activator, thus affecting sperm motility . When the prostate has inflammation, the citric acid content is significantly reduced. (4) Protein: Determined by the double urine method. The normal person is 3.6 ± 0.8 g / dl. The protein in semen is mainly from the seminal vesicle and prostate, which can promote sperm motility and protect sperm from environmental harmful effects. (5) Acid phosphatase: measured by the method of β-glycerophosphate. The normal value is 882 ± 412 Brine units / ml. The enzyme is mainly from the prostate. It is related to the vitality and metabolism of sperm. In prostatitis, the content of acid phosphatase in seminal plasma decreases; early prostate cancer or benign prostatic hypertrophy, acid phosphate enzyme content often increases. (6) Sperm-specific enzyme-Lactate dehydrogenase isozyme has relative tissue specificity and has a certain relationship with spermatogenic function. Not suitable for the crowd Inappropriate people: not yet known. Adverse reactions and risks No related complications or hazards.