Urine mucopolysaccharide detection

Urine mucopolysaccharide is detected by the aniline blue coloring method as a screening for mucopolysaccharidosis. Method: Collect morning urine, use a pipette to remove 0.1 ml of urine, drop it on the filter paper one by one, and make a round spot of about 6 cm; (after every drop of urine, blow dry with a hair dryer) will blow dry urine spots The filter paper is immersed in 0.2% toluidine blue dyeing solution (1 ml of toluidine blue plus 100 ml of distilled water, and then taken into 5 ml of acetone and 20 ml of acetone), dyed for 45 seconds, taken out and dried, and the dried dyed urine plaque filter paper is immersed in 10% acetic acid (glacial acetic acid 10m plus distilled water 90ml) soak for 4 minutes to decolorize, if not clean, then take off again, dry in the air. At the same time, normal human urine is used as a control. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The urinary plaque is purple-blue or punctate. Tips: Pay attention to the normal diet, pay attention to normal work and rest, and prevent endocrine disorders. Normal value The urinary plaque is purple-blue or punctate, and the normal urine urinary tract is colorless. Clinical significance Positive: Use cellulose acetate membrane electrophoresis to distinguish the type of mucopolysaccharide excreted in the urine to assist in typing. Abnormal results: normal development after birth, signs gradually appear before the age of one. Stunting after 1 year of age, skeletal deformities gradually become apparent. The head is large, the forehead is prominent, and the skull is a boat-like deformity. Short neck, lower chest and upper lumbar spine. The bridge of the nose is flat and wide, the lips are large and everted, the tongue is wide open, the teeth are sparse and small, and the gums are thick. The face is rough, the expression is indifferent, and the intelligence is backward. The hair is rough and black. Most have joint contractures, palms and fingers are wide and short, knees and hips are everted and flat feet. Abdominal bulging, hepatosplenomegaly or suffocation, normal liver function. Corneal cloud turbidity. Nasopharyngeal malformations, deafness, prone to otitis media and upper respiratory tract infections and pneumonia. Thoracic deformity. Due to the deposition of mucopolysaccharide in the vessel wall, coronary artery occlusion or myocardial infarction can occur. The people who need to be examined: the head is large, the forehead is prominent, and the skull is a boat-like deformity. Short neck, lower chest and upper lumbar spine. The nose is flat and wide, and the lips are large and the eversion is suspected of symptoms of mucopolysaccharidosis. Positive results may be diseases: pediatric mucopolysaccharidosis, pediatric mucopolysaccharide storage disease type IV, mucopolysaccharide metabolism disorder, pediatric glycogen storage disease type I, fucosidosis storage disease precautions Forbidden before examination: pay attention to normal diet, pay attention to normal work and rest, and prevent endocrine disorders. Requirements for inspection: Actively cooperate with the doctor's request. Inspection process Method: Collect morning urine, use a pipette to remove 0.1 ml of urine, drop it on the filter paper one by one, and make a round spot of about 6 cm; (after every drop of urine, blow dry with a hair dryer) will blow dry urine spots The filter paper is immersed in 0.2% toluidine blue dyeing solution (1 ml of toluidine blue plus 100 ml of distilled water, and then taken into 5 ml of acetone and 20 ml of acetone), dyed for 45 seconds, taken out and dried, and the dried dyed urine plaque filter paper is immersed in 10% acetic acid (glacial acetic acid 10m plus distilled water 90ml) soak for 4 minutes to decolorize, if not clean, then take off again, dry in the air. At the same time, normal human urine is used as a control. Not suitable for the crowd Inappropriate crowd: temporarily unknown. Adverse reactions and risks No obvious complications and harms.