Bile lysis test

The biliary lysis test is carried out according to the following principle. Bile or bile salts can dissolve S. pneumoniae, possibly due to bile lowering the surface tension of the cell membrane, causing cell membrane damage or lysis of the cells; or because bile accelerates S. pneumoniae itself. The autolysis process promotes autolysis of bacteria. Reagent 10% sodium deoxycholate or pure bovine bile. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The plate method was judged as "colony disappeared"; the test tube method was made transparent with the \\\\\\\" bile salt culture, while the control tube was still "turbid" and was positive. Tips: Pay attention to the normal diet, pay attention to normal work and rest, and prevent endocrine disorders. Normal value The biliary lysis test was negative. The plate method was judged as "colony disappeared"; the test tube method was judged as "the culture of the bile salt became transparent, while the control tube was still "turbid" was judged to be positive. If the positive reaction did not occur, it was a negative reaction. Clinical significance Mainly used for the identification of Streptococcus pneumoniae and Streptococcus aureus, the former is positive, the latter is negative. Abnormal results: 1. Streptococcus pneumoniae usually reside in the nasopharyngeal cavity of normal people, most of which are not pathogenic, and only a few are toxic. When the body's resistance is reduced, it can often invade the lung tissue and cause pneumonia. In addition, it can cause purulent meningitis. Streptococcal pneumonia, formerly known as lobar pneumonia (90% of out-of-hospital acquired pneumonia), typical symptoms are sudden chills, high fever, chest pain, cough and rust stains. 2. Streptococcus aureus This type of bacteria can attack the normal endocardium. The patient has long-term fever, chills, and progressive anemia. Some bacteria and blood substances (fibrin) deposit on the heart valve to form sputum of different sizes. The sputum can fall off and form emboli in the blood flow, leading to embolism. In particular, brain, spleen, and renal embolism are common, resulting in sputum, splenomegaly, and red blood cells in the urine. If the embolism occurs in the subcutaneous or mucous membranes, the skin and mucous membrane defects, Osler's nodule, and the formation and exfoliation of the neoplasms often change accordingly. Positive results may be diseases: acute cholangitis, Dubo histoplasmosis considerations Forbidden before examination: pay attention to normal diet, pay attention to normal work and rest, and prevent endocrine disorders. Requirements for inspection: Actively cooperate with the doctor's request. Inspection process method: (1) Plate method: Take a 10% sodium deoxycholate solution to inoculate a loop, add it to the colony of the tested bacteria, and observe the result after setting it at 35 ° C for 30 min. (2) Test tube method: 2 test cultures, 0.9ml each, add 10% sodium deoxycholate solution and normal saline (control tube) 0.1ml, shake and place 35 °C water bath for 10 ~ 30min, observe result. result: The plate method was judged as "colony disappeared" and the test tube method was judged as "the culture of the bile salt became transparent, while the control tube was still "turbid". Not suitable for the crowd Inappropriate crowd: temporarily unknown. Adverse reactions and risks No related complications or hazards.