Adrenocorticotropic hormone (ACTH) stimulation test

The adrenocorticotropic hormone (ACTH) excitatory test is an examination performed by an 18-hour intravenous drip method followed by urine free cortisol; 2 rapid method, blood test for cortisol. Polycystic ovary syndrome responded normally, while 17-ketosterol and 17-hydroxysterol were significantly elevated in patients with abnormal adrenal function. Basic Information Specialist classification: growth and development check classification: endocrine examination Applicable gender: whether men and women apply fasting: fasting Tips: When analyzing the results, pay attention to the influence of physiological factors, such as exercise, excitement, hunger, etc., can increase the secretion of adrenocorticotropic hormone. Normal value Before and after this experiment: 17-ketosterol and 17-hydroxysterol values ​​were normal and there were no symptoms associated with polycystic ovary syndrome. Clinical significance Reduction: seen in pituitary dysfunction, adrenal cortical tumors, pituitary tumors, pituitary damage. Elevation: seen in stress (such as burns, surgery, hypoglycemia, etc.), primary adrenal insufficiency, Cushing's syndrome, congenital adrenal hyperplasia, pituitary adrenocortical hormone cell tumor. Precautions (1) When high-dose glucocorticoids are treated, the results of adrenocorticotropic hormone measurement are also reduced. (2) When analyzing the results, attention to the effects of physiological factors, such as exercise, excitement, hunger, etc., can increase the secretion of adrenocorticotropic hormone. Inspection process All reagents except the precipitant should be equilibrated at room temperature before use. (1) Mark T (total count), NSB (non-specific binding), A (maximum binding) and B to G, indicating quality control and blood samples, all for double tube. (2) Add 100 μl of 0 standard to NSB and A tube, add 100 μl of corresponding reagent to B to G tube, and add 100 μl to both quality control and blood samples. (3) Add 100 μl of ACTH antiserum (except NSB and T tube) to each tube and mix. (4) Incubate for 60 min at room temperature. (5) 100 μl of ACTH marker was added to each tube. (6) Incubate at 4 ° C for at least 16 h. (7) Add 1 ml of a precipitant to each tube and mix. (8) Centrifuge at 3000 x g for 15 min. (9) The rack was tilted with a foam, the supernatant was poured, and the precipitate was retained for counting. (10) 1 min count. Note: Place the tube on the absorbent paper for 10 min, gently tap the tube to remove any remaining droplets. Not suitable for the crowd Inappropriate crowd: temporarily unknown. Adverse reactions and risks There are no related complications and hazards.