Histochemical staining of cells

Immunocytochemical staining is the use of known antibodies to bind to the specificity of cellular antigens. The chemically displayed reagents display a certain color on the antibody and are observed by means of a microscope, fluorescence microscope or electron microscope. The purpose of quantification and localization analysis of chemical components in tissues and cell structures. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The positive reaction has a gray flocculent precipitate in the cytoplasm, which has a differential value for acute mononuclear leukemia and other types of leukemia. Acute monocytic leukemia is weakly positive. Tips: Generally, the serum is taken on an empty stomach in the morning, and it is forbidden to check after eating and strenuous exercise. Normal value The test result was negative. Clinical significance Abnormal results: The positive reaction has gray flocculent precipitate in the cytoplasm, which has a differential value for acute mononuclear leukemia and other types of leukemia. Acute monocytic leukemia is weakly positive. There is a precipitation reaction, mainly used for the differential diagnosis of leukemia, leukemia, suppurative infection and myelofibrosis. The average positive cells of normal people are 20-40%; in purulent infection, leukemia-like reaction, bone marrow fibrosis alkaline phosphatase Increased, chronic myelogenous leukemia decreased. People who need to be examined: patients with leukemia and rheumatism need to be examined, and those with low immunity can. Precautions Forbidden before examination: Generally, the serum is taken on an empty stomach in the morning, and the diet is checked after eating and strenuous exercise. Requirements for inspection: pay attention to the serum is not contaminated, and do a fixed operation during the dyeing process. Inspection process The PAP antigen, antibody reaction and enzyme labeling principle are exactly the same as the APAAP method, and all belong to the immunohistochemical reaction, except that the labeled enzymes are different. The principle of PAP enzymatic chemical reaction is exactly the same as that of ABC-AP method, but the ABC-AP method is an affinity immunohistochemical reaction. The PAP method and the ABC-AP method are identical in the staining step and method. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks There are no related complications and hazards.