host-mediated test

The host-mediated assay is a test method for detecting genotoxicity of a test substance by using bacteria, fungi or mammalian cultured cells as indicator organisms, contacting poisons in whole mammals. The most commonly used microbial host mediation test is to give the test object by mouth, intramuscular or subcutaneous injection, and then intraperitoneally inject the indicator microorganism (such as Salmonella typhimurium or Escherichia coli mutant strain) and kill it after a certain period of time. Animals, go to the peritoneal lavage or isolate bacteria from the liver, plate, and culture to determine the number of viable bacteria and the number of bacteria. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a state of dynamic balance and health. Clinical significance Abnormal results: The pathogenicity of Staphylococcus aureus is mainly related to various invasive enzymes (such as plasma coagulase, hyaluronidase, phospholipase, catalase, thermonuclease) and various toxins (hemolytic toxins, killing Related to leukotoxin, etc., certain strains of enterotoxin can cause food poisoning, which is manifested as acute gastroenteritis. Among Streptococcus, Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus pneumoniae are three important pathogens that can cause suppurative tonsillitis, acute pharyngitis, iatrogenic infections, rheumatic fever, and small kidneys. Ball nephritis and respiratory infections. Infections caused by Enterococcus are most common in urinary tract infections, most of which are intra-hospital infections, often associated with urinary tract manipulation and catheterization, followed by trauma and postoperative infections in the abdomen and pelvis. Salmonella typhimurium enteritis (abbreviated as typhoid fever) is an acute infectious disease caused by Salmonella typhimurium. The clinical manifestations can be divided into four types, namely gastroenteritis type, sepsis type, lesion type and asymptomatic type. There are other infections caused by bacteria or fungi. The people who need to be examined are: rheumatic fever, sepsis, respiratory infection, fungal infection, acute gastroenteritis, urinary tract infection and other symptoms. Precautions Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process The most commonly used microbial host mediation test is to give the test object by mouth, intramuscular or subcutaneous injection, and then intraperitoneally inject the indicator microorganism (such as Salmonella typhimurium or Escherichia coli mutant strain) and kill it after a certain period of time. Animals, go to the peritoneal lavage or isolate bacteria from the liver, plate, and culture to determine the number of viable bacteria and the number of bacteria. The frequency of returning bacteria in each animal was calculated, and the average frequency of reversion of the combined control group was compared and statistically processed. The advantage of this assay is that the subject undergoes metabolic and kinetic processes in the body in vivo. Not suitable for the crowd Inappropriate crowd: No. Adverse reactions and risks There are no related complications and hazards.