Immunoturbidimetric technology

The early use of immunoturbidimetric technology was mainly used to determine the protein content in serum, urine and cerebrospinal fluid. The immunoturbidimetric technique utilizes soluble antigens and antibodies to specifically bind in the liquid phase to form a certain size of the antigen-antibody complex, so that the reaction solution exhibits turbidity. When the antibody remains excessive in the reaction solution, the formed complex increases as the amount of the antigen increases, and the turbidity of the reaction solution also increases, and the content of the sample can be calculated by comparing with a series of standards. At present, the immunoturbidimetric technology is mainly used for the detection of various proteins, apolipoproteins, haptens (such as hormones, poisons and various therapeutic drugs) and microorganisms. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Immunocompromised. Tips: Generally, the blood test is performed on an empty stomach in the morning, so it is forbidden to overeating and strenuous exercise. Normal value Generally it is a negative result. Clinical significance Abnormal results: 1. Immunological function monitoring immunoglobulin G, A, M, immunoglobulin light chain κ, λ, complement C3, C4 determination. 2, cardiovascular disease monitoring: apolipoprotein A, B, lipoprotein (a), C-reactive protein. 3, inflammatory status monitoring: C-reactive protein, a-acid glycoprotein, haptoglobin, ceruloplasmin and so on. 4, rheumatic disease detection: ASO, RF, CRP. 5, kidney function test: urine microalbumin, a-microglobulin, β-microglobulin, transferrin, immunoglobulin G and so on. 6, nutritional status monitoring: albumin, prealbumin, transferrin and so on. 7, blood coagulation and hemorrhagic disease detection: antithrombin III, transferrin, haptoglobin and so on. 8, blood-brain barrier monitoring: cerebrospinal fluid albumin, immunoglobulin G, A, M. People who need to be examined generally require immunocompromised persons to check, and newborns and pregnant women are also required to check certain items. Positive results may be diseases: adult rheumatoid arthritis scleritis, elderly rheumatoid arthritis precautions Taboo before the examination: Generally, the blood test is performed on an empty stomach in the morning, so it is forbidden to overeating and strenuous exercise. Requirements for inspection: pay attention to the influence of pseudo turbidity; the cause of pseudo turbidity formation is very complicated, mainly because the antiserum contains non-specific cross-reactive hetero-antibody components; the concentration of turbidity agent and reaction time are not well mastered; the turbidity treatment of the sample itself Improper; contamination and deterioration of reagents; equipment, especially cuvettes, etc. are not clean enough. Inspection process The immunoturbidimetric technique utilizes soluble antigens and antibodies to specifically bind in the liquid phase to form a certain size of the antigen-antibody complex, so that the reaction solution exhibits turbidity. When the antibody remains excessive in the reaction solution, the formed complex increases as the amount of the antigen increases, and the turbidity of the reaction solution also increases, and the content of the sample can be calculated by comparing with a series of standards. Not suitable for the crowd Inappropriate people: no special requirements. It is best not to check when taking immune drugs. Adverse reactions and risks There are no related complications and hazards.