Lymphocyte Turnover Test (LTT)

When T cells are cultured in vitro, stimulated by non-specific mitogens (such as Phytohemagglutimin, PHA) or specific antigens, the cell volume increases, metabolism is strong, protein and nucleic acid synthesis increases and can be split. Lymphocytes, the phenomenon of lymphocyte transformation. The level of lymphocyte transformation can reflect the level of cellular immunity. Therefore, the lymphocyte transformation test can be used as one of the indicators for measuring the immune function of the body. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: fasting blood test. Taboo spicy food and overeating. Normal value The morphological method lymphocyte transformation rate (LTT) was 60.1% ± 7.6%. Clinical significance Abnormal results: decreased lymphocyte transformation test, found in hepatitis B, hemorrhagic fever with renal syndrome (epidemic hemorrhagic fever), measles, cytomegalovirus infection, severe mycosis, severe tuberculosis, leprosy, systemic lupus erythematosus, Sexual liver parenchymal disease, immunodeficiency disease, etc. People who need to be examined: Patients with related diseases and immunocompromised persons can be examined. Positive results may be diseases: drug dermatitis, pediatric drug dermatitis considerations Taboo before the test: fasting blood test, taboo spicy food and overeating. Requirements for examination: observe lymphocyte transformation, do not stain too much, in order to observe the nucleolus. Strict aseptic processing is the key to the success of lymphocyte transformation assays. Inspection process Techniques for lymphocyte transformation assay: morphological examination. After co-cultivation with mitogens, the morphological changes of lymphocytes were observed under an oil microscope. Calculate the percentage converted to mother cells. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks There are no related complications and hazards.