Coombs' test

The Coombs test is also known as the antiglobulin test. Mainly used for the diagnosis of congenital hemolytic anemia and immune hemolytic disease in children. Even if an anti-IgG antibody is directly reacted with the erythrocytes of the patient to which the antibody has been bound (direct test), or Rh-positive red blood cells are reacted with the patient's serum (indirect test), agglutination may occur. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: No relevant information. Tips: Forbidden overeating and strenuous exercise before check-up, usually in the morning fasting test. Normal value The results after the test were negative. Clinical significance Abnormal results: commonly used to diagnose neonatal hemolytic disease and autoimmune hemolytic anemia. And for the determination of blood group antigens, the detection and identification of blood group antibodies, and the blood type compatibility test before transfusion. Positive in autoimmune diseases, drug immunity and alloreactive hemolytic anemia. People who need to be examined: routine examination before transfusion and follow-up after transfusion reaction, so both sides of the blood transfusion need to be examined. In addition, both newborns and pregnant women need to be examined. Positive results may be diseases: Raynaud's disease, Raynaud's disease considerations Taboo before the test: taboo overeating, and strenuous exercise, usually in the morning fasting test. Requirements for examination: When using the direct anti-human globulin microcolumn gel immunoassay to detect red blood cells in patients, it is necessary to use a suitable amount of EDTA in a test tube to collect blood samples. This specimen should not be stored in a refrigerator at 4 ° C -10 ° C to avoid red blood cells in the patient. False positive reaction caused by in vitro sensitization. Note that serum samples are not contaminated and can result in false positive results. Inspection process In the hemagglutination reaction, an antibody test method in which an anti-immunoglobulin antibody which allows antibodies to bind to each other is aggregated for observation when only the antibody does not cause aggregation or aggregation is not strong. The usual agglutination reaction is linked in the form of erythrocyte antigen-antibody-erythrocyte antigen to produce agglutination. The Coombs test is agglutinated in the form of red blood cell antigen-antibody-antiglobulin antibody-antibody-erythrocyte antigen. Not suitable for the crowd Inappropriate population: The human Coombs test during the treatment with cephalosporin antibiotics is directly positive, affecting the test results. Adverse reactions and risks There are no related complications and hazards.