Extractable Nuclear Antigen (ENA) Autoantibody Band Analysis

ENA is a non-histone acidic nucleoprotein particle composed of many small molecules of RNA and polypeptides in cells. ENA mainly includes antigens such as Sm, RNP, SSA, SSB, Jo-1, and Scb-70. Different autoimmune diseases can produce different anti-ENA antibodies. The detection of ENA antibodies is of great significance for the diagnosis and differential diagnosis of autoimmune diseases. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: No relevant information. Tips: Generally, the morning fasting test, avoid post-diet check, taboo spicy food and strenuous exercise. Normal value Normal reference value: negative. Clinical significance Abnormal results: 1. Anti-sm is a serum-labeled antibody of systemic lupus erythematosus (SLE), which is up to about 30% positive. 2. Anti-rRNP is another serum-labeled antibody of SLE with a positive rate of 10%. 3. The positive rate of anti-u1RNP in mixed connective tissue disease (MCTD) is over 95%. In SLE, this antibody positive is associated with the Raynaud phenomenon. 4. The positive rate of anti-SSA in Sjogren's syndrome (SS) is 60%. It can also be found in many other autoimmune diseases, including SLE (35%), scleroderma, polymyositis and rheumatoid arthritis. Disease, the positive antibody can also cause subacute cutaneous lupus lesions, and is also associated with SLE's extensive photoallergic dermatitis. IgG anti-SSA antibodies can cause neonatal lupus syndrome through the placenta, and individual antibodies and cardiac conduction systems Combined, can cause congenital heart block. 5. Anti-SSB is a serum-labeled antibody to Sjogren's syndrome, with a positive rate of about 40%. 6. Anti-Jo-1 is a serum-labeled antibody to polymyositis (PM) and dermatitis (DM) with a positive rate of 25% in PM. The antibody-positive patients often have symptoms such as myositis, interstitial lung disease and arthritis, which are easily misdiagnosed as chronic lung infection or rheumatoid arthritis. 7. Anti-Scl-70 is a serum-labeled antibody to systemic scleroderma (PSS) with a positive rate of 43%. 8.D`E is only seen in patients with MCTD. 9. Anti-DM-53 is only seen in patients with dermatomyositis (DM). 10. Anti-RA-54 is only found in patients with rheumatoid arthritis, with a positive rate of 14%. People who need to be examined: patients with low immunity and related diseases and symptoms can be tested, and elderly and newborns can be tested for physical examination. Positive results may be diseases: precautions for rheumatoid arthritis in the elderly Forbidden before examination: Generally, the morning fasting test, avoid post-dietary check, and taboo spicy food and strenuous exercise. Requirements for inspection: Pay attention to the fact that serum specimens are not contaminated and promptly sent for inspection. Inspection process There are various detection methods, such as immunoblotting (IBT), ELISA, convective immunoelectrophoresis, double diffusion, gold standard method and the like. The immunoblotting technique belongs to membrane carrier enzyme immunoassay, and the solid phase carrier is a nitrocellulose membrane adsorbed with a medical | educational network antigen. The basic principle is to extract ENA from calf or rabbit thymus, and then divide it into zones by SDS-PAGE electrophoresis, then transfer it to the nitrocellulose membrane, and add the serum to be tested (containing anti-ENA antibody) to the nitrocellulose membrane, anti-ENA antibody. Binding to the ENA antigen, forming an Ag-Ab complex, adding an enzyme-labeled anti-human IgG, forming an Ag-Ab-enzyme-labeled Ab complex, and then adding a substrate to develop color. Different anti-ENA antibodies can be identified by comparison with standard bands. The immunoblotting technique does not require purification of antigen, has high sensitivity, high specificity, and is easy to operate. It is a widely used technique for detecting anti-ENA antibodies. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks There are no related complications and hazards.