NK cell activity assay (NK)

Natural killer (NK) cells are a group of heterogeneous, multifunctional immune cells that are naturally toxic kill cells that are not associated with a specific immune response. It has a regulatory effect on a variety of cells in the body, especially T and B lymphocytes. Its mediated lysis cell action is not limited by the major tissue-compatible complexes. Natural killer cells also have a strong scavenging effect on cancer cells, viruses, intracellular parasites and aging mutant cells. The killing effect of NK cells does not require pre-immunization or sensitization, and the killing effect is earlier than other effector cells, which is the first line of defense against tumors. NK cells play a role in the pathogenesis of antiviral and autoimmune diseases, and have important significance in anti-tumor and immune regulation. Therefore, it has been found that enhancing the active compound of NK cells has important significance for immunomodulation and anti-tumor. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: Negative: 47.6-76.8 Above normal: negative: NK cell activity was measured normally. Positive: The NK cell activity of the test sample group was significantly higher than that of the control group, and the test result was judged to be positive. Increased activity, common in the early stages of viral infection, Down syndrome, patients undergoing organ transplantation, bone marrow transplantation, and immunopotentiators. Reduced activity, common in malignant tumors, severe combined immunodeficiency disease, AIDS and immunosuppressive therapy. Tips: Need to take a fasting blood test, taboo overeating and after intense exercise. Normal value Reference value NK cell activity (natural killing rate) 47.6% ~ 76.8%. The test result was negative. Clinical significance Abnormal results: The NK cell activity of the test sample group was significantly higher than that of the control group, and the test result was judged to be positive. Increased activity, common in the early stages of viral infection, Down syndrome, patients undergoing organ transplantation, bone marrow transplantation, and immunopotentiators. Reduced activity, common in malignant tumors, severe combined immunodeficiency disease, AIDS and immunosuppressive therapy. People who need to be examined: patients undergoing organ transplantation, bone marrow transplantation, etc., and patients treated with immunopotentiators need to be examined. Those with low immunity can also check. Positive results may be diseases: children with X-linked severe combined immunodeficiency disease, congenital tumors, early baldness matters needing attention Contraindications before the examination: need to take a fasting blood test, taboo overeating and after intense exercise. Requirements for examination: target cells and effector cells must be fresh, and cell viability should be greater than 95%. The ambient temperature should be kept constant during colorimetry. Inspection process YAC-1 cells (survival rate >95%) with good growth 24 h after passage were labeled with 1×106/mLYAC-1 cell suspension plus 3H-TdR10uCi, and cultured for 2 h at 37 ° C in a 5% CO 2 incubator. Oscillation once every 30 minutes. The labeled cells were washed three times with the culture solution, and resuspended in the culture solution to have a cell concentration of 1 × 10 5 /mL. This is followed by NK cell activity assays, followed by data processing and results determination. Not suitable for the crowd Inappropriate people: no special requirements. Adverse reactions and risks There are no related complications and hazards.