Prostatic fluid culture

Prostatic fluid culture is the most reliable method for diagnosing bacterial prostatitis. The selection of effective antibiotics through drug sensitivity test can improve the clinical treatment effect. Prostatic fluid collection should be abstinence for more than 5 days. Otherwise it will affect the results of the inspection. Antibiotics should be discontinued before specimen collection. The external genitalia should be carefully cleaned before collecting prostatic fluid specimens. Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: not fasting Tips: Prostatic fluid collection should be abstinent for more than 5 days. Antibiotics should be discontinued before specimen collection. The external genitalia should be carefully cleaned before collecting prostatic fluid specimens. Patients with lesions outside the urethra should not use the direct method to collect prostatic fluid. Normal value No colonies were found after prostatic fluid culture. The pH of normal prostatic fluid is 6.5-6.8, which is acidic. The pH of prostatic fluid in patients with chronic prostatitis can be increased to 8.0-8.2, which tends to be alkaline. Clinical significance Abnormal results: bacterial culture examination can find pathogenic bacteria, staphylococci are the most common, followed by streptococcus, gonorrhea can also be found. Mycobacterium tuberculosis can also be found in patients with tuberculosis. People who need to be examined: patients with bacterial prostatitis. High results may be diseases: prostatitis, chronic bacterial prostatitis, prostate cysts, gonorrhea complication prostatitis precautions Taboo before inspection: 1, prostate fluid collection should be abstinence for more than 5 days. Otherwise it will affect the results of the inspection. 2. Antibiotics should be stopped before specimen collection. Because, after the antibiotic enters the body, it can reach various tissues and organs in the human body in a short period of time, so that the growth and reproduction of microorganisms such as bacteria in the specimen can be inhibited, resulting in false negative results, and some pathogens are missed. Therefore, if the patient is receiving antibiotic treatment at the time of treatment, whether it is for the treatment of prostatitis or for the treatment of other organ infections, the specimens such as urine and prostatic fluid should be collected after 3 days of withdrawal. And routine inspection, isolation and culture of pathogens. Of course, this may not be necessary in certain special circumstances, such as acute prostatitis or because of some other special reasons. 3, the foreskin is too long can cause false positives. Because of the presence of a variety of bacteria or other microorganisms in the urethra, urethral mucosa, and penile skin of normal male urethra or prostatitis patients, specimens such as collected prostatic fluid are often contaminated by microbes present on the surface of the external genitalia. . These contaminated specimens of microorganisms can cause false positive results of bacteria such as the original sterile prostatic fluid, which makes people mistakenly believe that the patient's prostate or other organs of the reproductive system are infected or even seriously infected. Therefore, you must pay attention to cleaning the vulva before collecting the specimen, check whether the foreskin is too long, and whether there is infection. 4. The external genitalia should be carefully cleaned before collecting the prostate fluid specimen. Requirements for inspection: 1. The collected prostate fluid should be collected in a sterile bottle. 2. The specimen should be sent for inspection in time. Routine examination of chronic prostatitis, such as urine and prostatic fluid examinations, should be sent to the laboratory as soon as possible after collection of specimens. Because the specimen stays in vitro for too long, it is highly probable that the number of pathogens in the specimen will increase or decrease due to the death or growth of certain bacteria and other microorganisms, resulting in the analysis of false negative results or results that are inconsistent with the existing conditions. For example, after a urine specimen is left at room temperature for a long time, the pH of the urine is lowered due to the acidity of the urine itself or due to the metabolism of some bacteria, which may cause a large number of deaths of acid-sensitive bacteria, mycoplasma and the like. Missed or misdiagnosed. Specimens such as prostatic fluid, due to their rich nutrients, are placed at room temperature for a long time, which is beneficial to the growth, reproduction and metabolism of certain bacteria or microorganisms, resulting in an increase in the number of certain microorganisms in the specimens, and other microorganisms. The number is reduced, resulting in misdiagnosis or missed diagnosis. Inspection process Specimen collection: Prostate fluid collection methods can be roughly divided into direct method and indirect method. 1. Direct method: the silicone catheter is placed in the posterior urethra, the prostate is massaged, and the prostatic fluid is directly collected for bacterial culture. The perineum, the external urethra and the anterior urethra should be disinfected before collecting the specimen to avoid contamination. 2. Indirect method: The use of transurethral prostate specimens for bacterial culture and histology is an invasive procedure. The clinically practical and simple method is the positioning method, that is, the urine is taken in three steps, and each segment takes 10 ml, which is represented by VBl, VB2, and VB3, respectively. VB1 is the urine before the prostate massage, VB2 is the urine of the bladder, VB3 is the urine after the prostate massage, the urine for the bacterial culture is located with the prostate, and the EPS of the prostatic fluid is taken for bacterial culture. For example, the number of bacterial culture colonies of VB3 bacteria is more than 110 times higher than VB, indicating the presence of bacterial prostatitis. The advantage of this positioning method is that it is simple and easy to perform, and can simultaneously obtain bacterial culture specimens of urethra and bladder urine. Not suitable for the crowd Inappropriate population: Patients with lesions outside the urethra should not use the direct method to collect prostatic fluid. Adverse reactions and risks There are no related complications and hazards.