Sperm acrosome integrity test

The acrosome plays an important role in sperm fertilization. Because it releases the protein's decomposing enzyme, it digests the binding matrix between the cumulus cells. The sperm can be fertilized through the capsule of the egg. Malformation due to acrosome structure can result in reduced or no fertilization. Sperm aging or damage can cause damage to the acrosome cap. First, the acrosome ridge changes partially, and finally the entire acrosome disappears. Freezing and thawing of semen can damage some sperm, and it is important to know the percentage of injured sperm. Studies have shown that there is a greater correlation between the acrosome integrity rate and the non-return rate than the sperm survival rate and the non-return rate. Therefore, it is important to check the sperm acrosome integrity rate. Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: not fasting Tips: Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. Normal value The normal reference value for acrosome integrity in normal fertile men is >75%. Clinical significance Abnormal results sperm acrosome integrity rate <75%, suggesting male infertility. Male infertility patients need to be examined. Low results may be diseases: male infertility, male genital malformation precautions Forbidden before examination: Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. Requirements for inspection: 1. When taking semen, you can use soft soap or paraffin oil for penile massage, collect the specimens in sterile test tubes; use condoms (wash clean, no spermicides) or use semen interruption method to collect semen, but this way The amount collected is often small. 2. If the semen is not obtained by the above method, the seminal vesicle and the end of the vas deferens can be massaged through the rectum, and the urine can be collected to check whether there is sperm in the sediment. 3. Do not expose semen to overheated and cold conditions. Hand it to the doctor for reference, no more than 30 minutes. Keep warm in cold weather, and keep it in your underwear pocket when you send it. 4. If bacterial culture is to be carried out, the urethral opening should be rinsed and disinfected, and the semen should be collected in a sterile test tube. 5. If you are not giving birth, both husband and wife must go to check. Inspection process Analysis of sperm acrosome integrity requires smearing and staining of sperm and analysis of sperm morphology. According to the shape and damage of the acrosome, the sperm acrosome is divided into four types. Type I: The acrosome is intact, the sperm morphology is normal, the coloration is uniform, the acrosome edge is neat, and sometimes the clear equatorial plate is visible. Type II: The acrosome is slightly inflated, and the sperm plasma membrane (acrosomal membrane) is loosely enlarged. Type III: Acrosome destruction, the sperm plasma membrane is severely expanded and destroyed, the coloration is shallow, and the edges are not neat. Type IV: The acrosome is completely detached and the sperm nuclei are bare. Types II, III, and IV are all acrosomal incomplete sperm. When calculating the acrosome integrity rate, 200 sperms are generally counted, and the percentage of spermatozoa of type I acrosome is counted. Acrosome integrity rate (%) = acrosome intact sperm count / total sperm count × 100% Not suitable for the crowd The examination is less invasive and generally has no contraindications. Adverse reactions and risks This test is less invasive and generally does not cause serious complications or other hazards.