Two-way agar diffusion test

Two-way agar diffusion tests are often used for qualitative testing and for semi-quantitative testing. The antigen and the antibody are separately added to the adjacent pores on the agar gel plate, and they are allowed to diffuse toward each other. When the two meet at the most appropriate ratio, a clear sedimentation line is formed. Depending on whether or not a precipitation line is present, an unknown antibody can be identified using a known antibody, or an unknown antibody can be identified using a known antigen. This method is commonly used to examine alpha-fetoprotein in the serum of patients with primary liver cancer as an early auxiliary diagnosis of primary liver cancer. Basic Information Specialist classification: Digestive examination classification: liver function test Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: The result of the negative test should be normal. Positive: Positive test results suggest that you may have liver cancer. Tips: Taboo overeating and strenuous exercise. Especially diet-stimulating foods such as wine. Normal value The precipitation line close to the antigen hole indicates that the antibody content is large; close to the antibody well indicates that the antigen content is high; the absence of the precipitation line indicates that the antibody or antigen is lacking or the antigen is excessive, which is negative. In addition, if multiple precipitation lines appear, it means that neither the antigen nor the antibody is a single component. Therefore, it can be used to identify the purity of an antigen or antibody. Clinical significance Abnormal results The antigen-antibody in this experiment is not particularly fixed. Two-way agar diffusion test is often used for qualitative detection and semi-quantitative detection. Known antibodies can be used to identify unknown antigens, or known antibodies can be used to identify unknown antibodies. Different concentrations form different lines of precipitation. This method is commonly used to examine alpha-fetoprotein in the serum of patients with primary liver cancer as an early auxiliary diagnosis of primary liver cancer. People who need to be examined have liver cancer and related diseases who need to be examined, and long-term smokers need to check. Positive results may be diseases: liver cancer precautions Taboo before the test: taboo overeating and strenuous exercise. Especially diet-stimulating foods such as wine. Requirements for examination: fasting blood test. Inspection process The antigen and the corresponding antibody are separately added to adjacent wells in the same gel plate. The two diffuse each other and form a precipitate of the antigen-antibody complex when they reach the site where their concentration ratio is appropriate. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.