Fungal check

Fungal examination is a method of direct microscopy to find hyphae and spores for initial diagnosis. When collecting specimens, pay attention to the quality of the specimens, which directly affects the results of the examination. Therefore, the collection of multiple sites can increase the positive rate. When making the film, the overflowing sealant should be sucked away to avoid corrosion of the lens. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: A negative test result indicates that the body is in a normal state. Positive: A positive test indicates that the subject may have a fungal infection. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the body surface and the body flora are normal, and the human body is in a state of dynamic balance and health. Clinical significance Suitable for fungal infections in the shallow and deep parts. Direct microscopic examination, positive indicates the presence of fungi, but generally can not determine the species. Negative can not be completely excluded. Abnormal results Shallow fungi (flying fungi) only invade the skin, hair and nails, while deep fungi can invade human skin, mucous membranes, deep tissues and internal organs, and even cause systemic disseminated infections. Deep fungal infection in the intestine manifests as fungal enteritis, which can exist independently as infantile candidiasis enteritis, or one of the manifestations of systemic fungal infections, such as AIDS complicated by disseminated histoplasmosis. People who need to be examined: mucosal damage, deep tissue damage, systemic disseminated infection, candida enteritis, disseminated histoplasmosis and other symptoms. Positive results may be diseases: cutaneous candidiasis, athlete's foot, dermatophytosis, head lice, zygomycosis, body lice, athlete's foot, tongue disease, asbestos-like pityriasis, onychomycosis considerations When collecting specimens, pay attention to the quality of the specimens, which directly affects the results of the examination. Therefore, the collection of multiple sites can increase the positive rate. When making the film, the overflowing sealant should be sucked away to avoid corrosion of the lens. Keeping the infected area clean and dry helps to inhibit the proliferation of fungi and promote skin healing. The infected area should always be washed with soap and water, dried and then sprinkled with talcum powder. Avoid using powders containing corn flour because it promotes fungal growth. Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Direct microscopy [Collecting specimens] Taking specimens is the key to verifying that you can get the right results. The corresponding specimens were selected according to clinical manifestations. Scales, fungus: use a blunt knife to scrape the scales on the edge of the lesion. A disease: first remove the free dirt on the surface of the nail, take the dander under the deck near the nail bed. Pus, exudate and various secretions: Take directly with a cotton swab. Hair: Use a pair of tweezers to remove the diseased hair and hair loss that is tarnished and wound around the fungus. Cerebrospinal fluid, ascites, urine, should be centrifuged and take a precipitate. Actinomycosis: Look for sulphur-like particles. [Production] 1. Place the collected specimen on a glass slide, add 1 drop of 10% potassium hydroxide solution, and cover the coverslip. 2. Slightly warm, avoid boiling, expel the blisters, gently thin into a cloud, absorb the spill, first observe the hyphae and spores under a low magnification microscope, and then confirm with a high power microscope. [Mirror observation] (1) The hyphae are uniform in thickness under the microscope, green refraction, separation, uniform cytoplasm, and granules of different sizes in the cytoplasm. Most of the spores are round or oval, and the size is the same, and the internal structure of the spore is visible. (2) Ink smear: used to check cryptococcal and other capsule spores. The method is to take a small drop of ink and mix with the specimen (such as cerebrospinal fluid), and cover the cover glass directly after microscopic examination. (3) Smear or tissue section staining: Smear staining can better show the morphology and structure of the fungus. Gram stain is suitable for Candida albicans, spores, etc.; Wright's stain is suitable for histoplasma; tissue sections are usually stained with PAS, and most fungi can be dyed red. Not suitable for the crowd The test is a non-invasive test with no specific contraindications. Adverse reactions and risks This test is a non-invasive test and does not cause serious complications or other hazards.