Bacterial morphological examination method

The method of morphological examination of bacteria is to observe the morphology of the bacteria and the morphology of the flora to obtain the type of bacteria to be examined and the method of inspection of which genus belongs to. The thickness of the smear. The degree of fixation. Rinse the method, do not face the smear area. Pay attention to normal eating habits before the examination, pay attention to personal hygiene. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative results suggest no pathogenic microbial infection. Positive: Positive results suggest that there may be pathogenic microbial infections. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a state of dynamic balance and health. Clinical significance Check the infected site to determine the type of bacteria that cause the infection, and then treat the drug in a more targeted manner. Abnormal results: (1) The main symptoms of gonococcal infection of gonorrhea include frequent urination, urgency, dysuria, purulent discharge in the urethra, cervix or vaginal opening. Or gonococcal conjunctivitis, enteritis, pharyngitis, etc., or disseminated gonorrhea symptoms. (2) C. neoformans is an important opportunity for respiratory infections. It can cause serious clinical symptoms, most commonly meningitis. (3) Diphtheria bacilli invade the body and grow only in the throat, which produces diphtheria toxin into the blood, which can cause symptoms such as myocarditis and adrenal dysfunction. (4) Symptoms of infection caused by other pathogens. Patients with bacterial infections such as Neisseria gonorrhoeae, Cryptococcus neoformans, and Corynebacterium diphtheriae that need to be examined. Positive results may be diseases: diphtheria, diphtheria myocarditis, conjunctivitis, acute gonorrhea, acute pseudomembranous candida stomatitis considerations Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process The tissue of the infected part is taken for culture, and the culture solution is taken for examination. Morphological examination classification First, do not stain specimen inspection Hanging drop method Pressure drop method. Second, staining specimen inspection Single staining method (Mulan staining); Counterstaining method (Gram dyeing, acid-fast staining); General procedure for staining specimen inspection. First, smear preparation Smear - dry - fixed; Second, dyeing Initial dyeing - mordant - bleaching - counterstaining. Not suitable for the crowd The test is a non-invasive test with no specific contraindications. Adverse reactions and risks Risk of infection: If you use an unclean device to take the part of the test, there is a risk of infection.