Mircaria hatching method

The buttercup hatching method is an auxiliary inspection method for detecting parasites. The schistosomiasis patients have fewer eggs in the feces, and the direct smear method is not easy to detect. The scorpion hatching method is most often used in combination with the natural sedimentation method or the nylon sieving method for the diagnosis of schistosomiasis infection. The oocysts of schistosomiasis in the water at 25~28°C and pH 7.5~8.0 can hatch in a short time. After hatching, the edulis move in a straight line close to the water surface. Because of this method, a large amount of feces is concentrated by water washing natural sedimentation method or by using nylon sieving and sifting eggs, and then hatching is carried out, so that the detection rate is significantly improved compared with the general method. This phase check can be used to determine the corresponding symptoms. Basic Information Specialist classification: Digestive examination classification: feces / parasitic examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: A negative check value indicates no parasitic infection. Positive: A positive reaction indicates a parasitic infection. Tips: Do not take anti-parasitic drugs before inspection, so as not to affect the results. Normal value No parasite eggs or other pathogens were found to be negative during the examination. Clinical significance Abnormal results detected parasite eggs or other pathogens. People who need to be examined have abnormal gastrointestinal tracts. Positive results may be diseases: schistosomiasis, schistosomiasis and intestinal diseases Contraindications before the test: Do not take anti-parasitic drugs before the test, so as not to affect the results. Requirements for inspection: 1. The stool must be fresh and must not be mixed with urine. When the temperature exceeds 26 °C, the hatchling rate is greatly reduced after the stool is left for 24 hours, and the buttercups cannot be hatched after 48 hours. Therefore, if the feces can not be hatched in time, physiological saline can be added, and the suspension can be adjusted to be placed in a refrigerator at 4 ° C for 1 to 2 days without affecting the hatching rate. 2. The optimum temperature for hatching is 25~28°C. Below 10°C or above 30°C, the hair is not easy to hatch. 3. In summer and autumn, the temperature is high. In order to prevent the natural sedimentation process, the buttercups are hatched too early, and 1.2% saline can be used instead of clear water to inhibit the hatching of the buttercups. However, the dechlorinated tap water is still needed for hatching. 4. There are protozoa in the river water and pond water. The shape and dynamics are very similar to the edulis. It is easy to be misdiagnosed. Therefore, when the mites are hatched, the sedimentation and hatching water should be treated to kill the protozoa in the water. Although there are no protozoa in the tap water, there is often too much residual chlorine, which is unfavorable for hatching, and it needs to be determined by residual chlorine and dechlorination. Inspection process Pour the fecal sediment collected by natural sedimentation method or nylon sieving method into the incubator, add pH water (about 30 ° C water temperature) to the bottle neck, and then move the incubator to 25 ° C. Incubate the buttercups under conditions. After about 4 to 8 hours of incubation, remove the hair follicles. If they are negative, continue to incubate. Check again after 8 to 10 hours and 20 to 24 hours. If they are still negative, the report is negative. Face the light source during the inspection, place the hatching bottle on a black background, observe with the naked eye or enlarged mirror, and look at the eyes with both eyes. Pay attention to the small white spots that move quickly near the water surface of 1 cm. See the tip size, diamond shape, milky white, Translucent white spots, while carefully observing the movement characteristics of these small white points (straight line swimming, hitting the wall quickly), that may be the donkey. Special attention should be paid to the identification of other protozoa (such as paramecium) in the water. If the visual observation is difficult, the small white spot of the movement can be sucked out by a straw, placed on a glass slide, and identified by a low power microscope. The basic morphological characteristics are: pear shape, and cilia on the body surface. If no edulis is found, the slag in the hatching bottle can be sifted through 260 mesh, and the smears of the slag can be sucked up under the microscope to find the eggs. This can significantly improve the detection rate (may have some eggs in the ranunculus) Stunted and unable to hatch). Not suitable for the crowd The test was non-invasive and had no specific contraindications. Adverse reactions and risks The test is non-invasive, free of complications and other hazards.