polymerase chain reaction technology

The polymerase chain reaction technique for diagnosing the presence of Helicobacter pylori requires only a small amount of DNA, and does not require the presence of viable bacteria (this is different from other H. pylori detection methods). It can not only detect Helicobacter pylori in the stomach, but also hope to detect it. Outside the stomach, such as plaque, Helicobacter pylori in feces, can also be used for epidemiological investigations, its sensitivity far exceeds other methods, is conducive to determine the source and route of bacterial infection, it is a very Promising detection method for Helicobacter pylori. Basic Information Specialist Category: Digestive Examination Category: Genetic Testing (DNA) Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: A negative result indicates that the body is normal. Positive: A positive result suggests a peptic ulcer or chronic gastritis. Tips: You should not eat any more after dinner on the day before the check. You should not drink any water in the morning of the check. Normal value The mRNA (messeng ribonucleic acid) in which Hp has no related virulence factor gene is determined to be negative. Clinical significance Abnormal results were determined by the presence of Hp-associated virulence factor gene mRNA (messenger ribonucleic acid). Need to check the population of peptic ulcer, chronic gastritis patients. Positive results may be diseases: gastric injury, duodenal ulcer, acute suppurative gastritis, paralysis of hemolytic colitis, gastric perforation, gastric cancer, gastric bleeding, stomach, duodenal ulcer bleeding considerations Taboo before inspection: 1. Avoid smoking the day before the test to avoid intubation due to cough during the examination. 2. To have adult relatives and friends to accompany, remove the dentures before surgery. 3. Check that the day before dinner should not eat again, check the morning should not drink water. Taboo when checking: 1. Tell your doctor about your past medical history and history of drug allergies. 2. The patient should not do too much swallowing during the operation to prevent saliva from inhaling the airway and causing pain in the throat. 3. Explain to the patient the purpose of the gastroscopy, encourage the patient to establish confidence, not to be nervous, and cooperate with the doctor to successfully complete the examination. Take a gastric mucosal examination. 4. Prepare the gene fragment to ensure that the electrophoresis is completed. Inspection process 1. Materials: The specimens were collected from gastro-intestinal ulcers confirmed by gastroscopy, all kinds of gastritis and ulcers with gastritis, and 2 gastric mucosa were removed within 5 cm from the pylorus (two weeks before each examination) Anti-HP drugs were used for PCR and pathological biopsy to detect HP. 2. Research methods: PCR technology: HPDNA PCR diagnostic kit produced by Xiamen Great Wall Bioengineering Co., Ltd., DNA extraction using water boiling method, primers are urease gene fragments, and the acid sequence is: primer 1 is 5'- AAGCTTTTATTAGGGGTTT-3', primer 2 is 5'-TTATTACGCTTC-3', amplification product is 456bP, pre-denaturation at 94 °C for 5 minutes, 94 °C for 30 seconds, 55 °C for 30 seconds, 72 °C for 1 minute for a total of 32 cycles, 72 °C The extension was carried out for 5 minutes, electrophoresis of 2% agarose gel for 20 minutes, and BE staining showed positive bands at 456 bp, and was determined according to the brightness of the positive bands. Not suitable for the crowd Special diseases: Patients with hematopoietic dysfunction, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is necessary, try to avoid this examination. Adverse reactions and risks Risk of infection: If you use a dirty probe, you may be at risk of infection.