Free protein S assay

The free S protein assay is a disease diagnosis by measuring the amount of free S protein in the human body. The free S protein is an alpha single-chain glycoprotein in serum with a molecular weight of 83 kDa. The main regulation of SP is to compete with the metastable binding sites of C5b-7 for target cell membrane lipids, and to form membrane-bound C5b-7 by forming hydrophilic SPC5b-7 (abbreviated as S5b-7) complex. active. In this way, cells adjacent to the activation site of the complement can be protected from accidental attacks. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether women are fasting: fasting Analysis results: Below normal: Can be seen during pregnancy. Normal value: Normal value: 20-25mg/L Above normal: Can be seen in the inflammatory response. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value 20 ~ 25mg / L. Clinical significance Abnormal result PS is a cofactor for the activation of PC. It is a macromolecular VK-dependent protein. About 60% binds to C4b. Only free PS acts as a cofactor for APC, inactivating coagulation factors Va and VIIIa. PS deficiency accounts for 0.003% of the normal population. In the incidence of thrombosis accounted for 5 to 8%, patients with hereditary PS deficiency may have deep vein thrombosis and arterial thrombosis, PS deficiency may also occur in pregnancy, oral contraceptives, acute inflammation and anti-VK medication, PS content can be Dropped to 20%. People who need to be examined have red, swollen, hot, painful and dysfunctional people. Low results may be diseases: Pregnancy vomiting precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process The assay was performed simultaneously with two serum samples and Hb standard. Colorimetric colorimetry was performed at 10 min, 30 min, 50 min, 60 min and 80 min after color development. Linear range: The Hb standard solution was made into 100, 200, 300, 400, 500, 600 mg/L, and the linear relationship was observed by a 721 spectrophotometer. This method has a good linear relationship within 400 mg/L. When the sample content is higher than 400mg/L, it should be diluted and redone. After 10 minutes, the colorimetric, wavelength 500nm, distilled water zero, the result is calculated as follows: Serum free hemoglobin (mg/L) = [Ua-Ba1(Sa-Sa×0.1)-Ba]×100. Ua: Measuring tube optical density Sa: Standard tube optical density Ba: blank tube optical density. Precision: Three replicates of unknown serum samples were used for 10 replicates within and between batches. The results are shown in Table 3, showing good precision. Recovery experiment: 0.02 ml of a standard Hb solution having a content of 50 mg/L, 100 mg/L, and 200 mg/L was separately added to 0.2 ml of serum containing Hb 61.29 mg/L. The result was 97.58% to 101.11%, and the average recovery was 99.69%. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.