Diethyl ether emission test

The ether dispersion test is a method of eluting antibodies from red blood cells by using diethyl ether, and is mainly used for the release test of various IgG antibodies on red blood cells. It is mainly used for direct anti-positiveness caused by warm autoantibodies (IgG) or alloantibodies, and for isolating IgG antibody mixtures. Its greatest advantage is that it is suitable for the examination of acquired hemolytic anemia. The identification of Rh antibody is the best and should not be used for ABO antibody identification. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative results are generally normal. Positive: The subject is prompted to have a blood disorder. Tips: Pay attention to rest and ban smoking. Normal value No abnormal antibodies were found in the dispersion results and were negative. Clinical significance Abnormal result The antibodies dispersed from the experiment can assist in the diagnosis of abnormalities of various IgG antibodies on red blood cells. Need to check the crowd: blood disease patients, newborns. Positive results may be diseases: chronic disease anemia, anemia considerations Inappropriate crowd: people with a small number of red blood cells Taboo before the test: pay attention to rest, no smoking. Newborns are required to be sensitive to pain during the examination and need to be appeased during the blood draw. Inspection process 1. Take 1 part of the washed packed red blood cells, add 1 part of brine and 2 parts of diethyl ether. 2. Tighten the stopper and shake vigorously for 1 minute. Remove the stopper several times to expel the volatile ether. 3. Centrifuge at high speed for 10 minutes. After centrifugation, it is divided into 3 layers. The upper layer is ether, the middle layer is red blood cell matrix, and the lower layer is antibody and detection antibody. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.