Oxidative Acetate AS-D Naphthol Esterase Staining

The oxidized acetic acid AS-D naphthol esterase staining is a staining of blood samples using oxidized acetic acid AS-D naphthol esterase to help identify the type of acute leukemia. Alpha-naphtholacctateesterase (α-NAE), also known as non-specific esterase (NSE), can hydrolyze α-naphthol in matrix solution to produce α-naphthol, and then The nitrogen dye is coupled to form an insoluble colored precipitate that is localized in the cytosol. The differentiation direction was confirmed by electron microscopic observation and flow cytometry through benzidine, naphthol AS-D chloroacetic acid acetate and Giemsa-Rayleigh staining. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Negative reactions are generally normal. Positive: Positive reactions are common in leukemia. Tips: Inappropriate people: people with obvious bleeding tendency. Normal value Generally negative is normal. 1. The granulocyte system is a negative reaction or a positive reaction, and the positive reaction from promyelocytes to mature neutrophils is generally (+) ~ (++). This reaction is not inhibited by sodium fluoride. . 2. Mononuclear cell system The primary monocytes are negative or positive, and both young monocytes and monocytes are positive, generally (++) ~ (+++), and this reaction can be sodium fluoride. inhibition. 3. Lymphocyte lymphocytes are weakly positive, and this reaction is not inhibited by sodium fluoride. 4. The early red blood cells of the red blood cell system can be positive, and the degree of positive reaction with the cells gradually weakens, and this reaction is not inhibited by sodium fluoride. 5. Megakaryocytes and platelets are positive. Clinical significance Abnormal result Positive staining results in the appearance of brown-black particles in the cytoplasm. 1 (1) No particle precipitation, 0 points. 2 (±) particles are small, sparse, 0.5 points. 3 (+) granule precipitation occurred in the 1/2 region of the cytoplasm, which was 1 point. 4 (++) Particle precipitation occurred in the 3/4 region of the cytoplasm, which was 2 points. 5 (+++) The cytoplasm is covered with particles and is 3 points. 6 (++++) The cytoplasm is filled with dense particles, which is 4 points. In acute myeloid leukemia, leukemia granulocytes can be positive, and this reaction is not inhibited by sodium fluoride. In acute monocytic leukemia, leukemia primordial mononuclear cells can be positive, and young monocytes and monocytes are positive. The people who need to be examined: weak, pale, and have a sickness. Loss of appetite and weight loss. Easy to bruise or hemorrhage, sore throat, bronchitis with headache, low fever, mouth pain and rash. Lymph nodes, especially in the throat, underarms, and groin. A person who feels uncomfortable under the left flank. Positive result may be disease: leukemia precautions Inappropriate people: People with a significant tendency to bleed. Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Alpha-naphtholacctateesterase (α-NAE), also known as non-specific esterase (NSE), can hydrolyze α-naphthol in matrix solution to produce α-naphthol, and then The nitrogen dye is coupled to form an insoluble colored precipitate that is localized in the cytosol. The differentiation direction was confirmed by electron microscopic observation and flow cytometry through benzidine, naphthol AS-D chloroacetic acid acetate and Giemsa-Rayleigh staining. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.