erythrocyte serum total bilirubin

Bilirubin is a metabolite of iron-containing porphyrin compounds such as hemoglobin and myoglobin in the body. Bilirubin in serum does not bind bilirubin and binds bilirubin. When liver cell damage, bile duct obstruction, increased red blood cell destruction, or shortened lifespan, bilirubin metabolism often occurs abnormally, and jaundice appears clinically. The bilirubin metabolism test is mainly used to understand the presence or absence of jaundice, and to identify its type. Basic Information Specialist classification: Digestive examination classification: liver function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in aplastic anemia, chronic nephritis anemia and other diseases. Normal value: Normal value: 2-20μmol/L Above normal: Found in hemolytic jaundice, blood group incompatibility, falciparum malaria and neonatal jaundice. negative: Positive: Tips: Bilirubin is sensitive to light, and standards and specimens should be protected from light. Normal value Serum total bilirubin 2 ~ 20μmol / L (0.1 ~ 1.2mg / dl). Clinical significance 1. Determine whether or not jaundice and its degree: STB17.1～34.2μmol/L is recessive jaundice; 34.2～171μmol/L is mild jaundice; 171～342μmol/L is moderate jaundice;>342μmol/L is severe jaundice . 2. To judge the nature of jaundice: 342～513μmol/L for complete obstructive jaundice, 171～266.8μmol/L for incomplete obstruction, 17.1～119.7μmol/L for hepatic jaundice, and <85.5μmol/L for hemolytic jaundice. . 3, bilirubin classification to identify jaundice type of total bilirubin and unconjugated bilirubin increased, found in hemolytic jaundice, blood group incompatibility transfusion reaction, falciparum malaria and neonatal jaundice, etc., mainly unconjugated bilirubin; Total bilirubin and combined bilirubin are obstructive jaundice, such as cholelithiasis, liver cancer, pancreatic head cancer, etc., combined with bilirubin increase; total bilirubin, bilirubin, unconjugated bilirubin Increased to hepatocellular jaundice, such as acute jaundice hepatitis, chronic active hepatitis, cirrhosis and acute yellow liver necrosis. 4, for the degree of liver cell damage and prognosis in the liver disease, bilirubin concentration is significantly increased, often reflecting severe liver cell damage, poor prognosis. However, a small number of subacute hepatitis can occur without jaundice. Cholestatic hepatitis, serum bilirubin is very high, and liver cells are less damaged. 5, help to understand the severity of neonatal hemolysis, and develop a reasonable treatment plan. 6, aplastic anemia, chronic nephritis anemia patients with STB reduction. The result is low, the disease may be high: the result of high liver failure in children may be diseases: neonatal jaundice, aplastic anemia, pediatric acute cholecystitis Before the examination: symptoms of yellow skin, yellow urine and yellow eyes appear. The skin of the newborn and the sclera appearing within 24 hours are called yellow neonatal physiological jaundice. After the examination: Do not rely solely on drugs for treatment, diet should also pay attention to, develop a good diet, not only can relieve the disease, but also can cooperate with drug treatment to inhibit the development of the disease. Inspection process 1. Modified caffeine (JG) method: operate as required. After fully mixing, the wavelength is 600nm, the control tube is zeroed, and the absorbance of each tube is read; or the zero is adjusted with distilled water, and the absorbance of the measuring tube and the control tube is read, and the difference between the absorbance of the measuring tube and the absorbance of the control tube (AU-AC) is used. The corresponding bilirubin concentration was determined on the standard curve. 2. Bilirubin oxidase method: Operate as required. Immediately after adding the BOD solution, the mixture was placed in a 37 ° C water bath for 5 min, and the distilled water was adjusted to zero at a wavelength of 450 or 460 nm by a spectrophotometer, and the absorbance of each tube was read. The cuvettes for the control tubes and the cuvettes for the non-control tubes should not be mixed. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.