Serum sialic acid assay

Serum sialic acid is determined by measuring the amount of sialic acid in serum. Serum sialic acid is an important component of cell membrane glycoprotein, which is related to many biological functions of organisms, and is associated with cell malignant transformation, cancer metastasis, infiltration, loss of contact. Inhibition, decreased cell adhesion, and tumor antigenicity are closely related. Determination of serum sialic acid concentration, can be used as a diagnostic indicator for cancer diagnosis and efficacy indicators. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Normal value 331 ± 43.9 mg / L. Clinical significance Abnormal result The acutely elevated SA diseases include acute leukemia, esophageal cancer, cardiac cancer, gastric cancer, intestinal cancer, liver cancer, lung cancer, and ovarian cancer, among which acute leukemia patients are the highest. Increased serum sialic acid in tumor patients may be reflected in the increase in tumor cell glycoprotein synthesis and metabolic changes in the blood. The people who need to be examined: weak, pale, and have a sickness. Loss of appetite and weight loss. Easy to bruise or hemorrhage, sore throat, bronchitis with headache, low fever, mouth pain and rash. Lymph nodes, especially in the throat, underarms, and groin. A person who feels uncomfortable under the left flank. High results may be diseases: acute leukemia, gastric cancer, intestinal cancer precautions Inappropriate people: People with a significant tendency to bleed. Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.