Serum β-hydroxybutyric acid determination

Serum β-hydroxybutyric acid is a measure of serum β-hydroxybutyrate in human serum ketone bodies for the diagnosis of diabetic ketoacidosis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Normal value Blood β-hydroxybutyric acid <0.27 mmol/L. Clinical significance Abnormal result Blood or urine ketone body positive is more common in diabetic ketoacidosis, in addition to pregnancy vomiting, long-term hunger, malnutrition, strenuous exercise or taking biguanide hypoglycemic agents (DBI, D860). The people to be examined have loss of appetite, nausea, vomiting, and vomiting after drinking water. Diabetes, increased urine output, fatigue and fatigue, but no significant eating diabetes. High results may be diseases: Diabetes precautions Inappropriate people: People with a significant tendency to bleed. Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Establish a method for detecting the metabolism of D-3 hydroxybutyrate + acetoacetate in vivo, and establish a method for simultaneously determining the composition of two ketone bodies in the same reaction system in vitro, and perform detection on an automatic biochemical analyzer. The total amount of ketone bodies in the blood is determined to be 98%, which is closer to the true value of the ketone body, and is applied to the clinic to provide more accurate data for diabetic ketoacidosis. The establishment of this method is scientific, feasible and advanced. The rate of increase in NADH absorbance at 340 nm was measured by enzyme kinetics at pH 8.5, which is proportional to the concentration of BHB. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.