Plasma tissue plasminogen activator inhibitor activity assay

The detection of plasma tissue plasminogen activator inhibitor activity is carried out by measuring the activity of plasma tissue plasminogen activator inhibitor in human body and detecting by chromogenic substrate method. Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Normal value 0.1 to 1.0 inhibition unit/ml. Clinical significance Abnormal result Increased test results: common in prethrombotic conditions and thrombotic diseases. Reduced test results: common in primary and secondary fibrinolysis. According to the results of PAIA and PAIAg, PAI-1 defects can be classified into CRM+ type and CRM-type. The elderly population in the population to be examined, with limb pain, swelling, superficial venous engorgement and cords that can be touched along the vein. Low results may be diseases: high results of hemolytic jaundice may be diseases: thrombosis considerations Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Take the standard and prepare the standard solution with the effective price (U/mL) of 5, 6, 7, 8, 9, and 10 with physiological saline. Take another 6×10cm plastic test tube, add 450μl of 0.1% fibrinogen solution, preheat for 5min in 37°C water bath, and then take 50μl of each of the above six concentrations of standard solution preheated at 37°C. Add to each of the above test tubes, immediately time and shake well, and record the coagulation time. Each concentration was measured 2 times, averaged, and a standard curve was made. The sample to be tested is diluted with physiological saline to a suitable concentration, 50 μl is taken, and measured in parallel according to the measurement method of the standard curve twice, averaged, and the titer of the sample is calculated according to a standard curve or a regression equation. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.