Plasma tissue plasminogen activator activity assay

The detection of plasma tissue plasminogen activator activity is a measure of the activity of plasma tissue plasminogen activator in humans, and its determination can help determine the activity of fibrinolytic system. Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value 1.19--2.61U/ml. Clinical significance Abnormal result 1. Pathological increase: It shows that the ability to activate plasminogen is enhanced, and the activity of fibrinolytic system is increased, which is mainly found in hyperfibrinolysis and DIC. 2. Pathological reduction: insufficient ability to activate plasminogen, decreased activity of fibrinolytic system, mainly seen in prethrombotic state and thrombotic diseases, such as myocardial infarction, angina pectoris, cerebrovascular disease, diabetes, pregnancy-induced hypertension, deep Venous thrombosis, nephrotic syndrome, etc. The elderly population in the population to be examined, with limb pain, swelling, superficial venous engorgement and cords that can be touched along the vein. Low results may be diseases: diffuse intravascular coagulation, pregnancy-induced hypertension, mucopolysaccharide metabolic disorders Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Take the standard and prepare the standard solution with the effective price (U/mL) of 5, 6, 7, 8, 9, and 10 with physiological saline. Take another 6×10cm plastic test tube, add 450μl of 0.1% fibrinogen solution, preheat for 5min in 37°C water bath, and then take 50μl of each of the above six concentrations of standard solution preheated at 37°C. Add to each of the above test tubes, immediately time and shake well, and record the coagulation time. Each concentration was measured 2 times, averaged, and a standard curve was made. The sample to be tested is diluted with physiological saline to a suitable concentration, 50 μl is taken, and measured in parallel according to the measurement method of the standard curve twice, averaged, and the titer of the sample is calculated according to a standard curve or a regression equation. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.