Plasma prothrombin fragment 1+2 detection

Plasma prothrombin fragment 1+2 is detected by measuring the prothrombin fragment 1+2 status for the diagnosis of prethrombotic conditions and thrombotic diseases and for timely treatment. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Generally should be normal. Normal value: Plasma prothrombin fragment 1+2: 0.67-±0.19nmol/L Above normal: Common in prethrombotic states and thrombotic diseases. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value 0.67 ± 0.19 nmol / L. Clinical significance Abnormal result Increased F1+2 is seen in prethrombotic states and thrombotic diseases, DIC, DVT, acute leukemia (especially acute promyelocytic leukemia), hereditary AT-III deficiency, protein C deficiency, and protein S deficiency. People who need to be examined have spontaneous, minor injuries, and people who have a tendency to bleed for a long time after surgery. High results may be diseases: precautions for disseminated intravascular coagulation and thrombosis Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process 1. The collected plasmas are numbered 1 to 5, and each is divided into 2 parts, one of which is subjected to isoelectric precipitation to extract prothrombin, and the other is extracted by cesium citrate adsorption method. 2. Isoelectric precipitation method. The plasma was diluted 10 times with distilled water, the pH was adjusted to 5.3 with 5% glacial acetic acid, left at 4 ° C overnight, centrifuged at 3000 r / min for 15 min, the supernatant was discarded, and the precipitate was dissolved in physiological saline and filtered for use. 3, bismuth citrate adsorption method. Solution A was added to the plasma at a volume ratio of 10:1, stirred at 4 ° C for 30 min, allowed to stand for 30 min, centrifuged at 3000 r/min for 30 min, and the supernatant was discarded. The precipitate was washed with solution B, centrifuged at 3000 r/min for 15 min, and the supernatant was discarded. Repeat the wash 3 times. The precipitate was desorbed with solution C, stirred at 4 ° C for 0.5 to 1 h, and centrifuged at 3000 r / min for 15 min to take the supernatant. The supernatant was dialyzed against Solution D overnight at 4 °C. The supernatant was centrifuged at 3000 r/min for 15 min, which was a prothrombin solution. Not suitable for the crowd 1. Patients taking drugs such as oxidative drugs and steroid hormones may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.