Plasma Thrombin Detection

Plasma thrombin assay refers to the determination of thrombin in human plasma by automated hemagglutination analyzer, which generally includes plasma thrombomodulin antigen assay (TMAg), plasma thrombomodulin activity assay (TMA), plasma thrombin. The original fragment 1+2 is detected (F1+2). Plasma thrombin testing can be known for the presence of hypofibrinogenemia, tiny clots, and calcium ions. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally should be normal. Positive: Indicates the presence of plasma thrombomodulin antigen, possibly with diseases such as diabetes and microangiopathy. Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The plasma thrombin regulatory protein activity assay activity level was 16 μg/L. The plasma thrombin regulatory protein antigen was negative. Plasma prothrombin fragment 1+2 was detected at 0.67 ± 0.19 nmol/L. Clinical significance Abnormal result Plasma thrombin regulatory protein antigen test: Positive test results indicate the presence of plasma thrombomodulin antigen, suggesting further examination of LE protein. Positive clinical kidney damage, diabetic complications and microvascular disease. Detection of plasma thrombin regulatory protein activity: analysis of activation of protein C by thrombin to activated protein C (PCa) in the presence of a thrombin cofactor, i.e., the thrombin regulatory protein, in a biological medium from a biological sample The method comprises adding to the plasma sample the reagent required for activation of the protein C system, the addition of purified protein C, and also the addition of a fibrin polymerization inhibitor. Detection of plasma prothrombin fragment 1+2: Explore the changes of disseminated intravascular coagulation (DIC) and fibrinolysis by prothrombin fragment 1+2, and guide its early diagnosis and treatment. People who need to be examined: middle-aged and elderly people, with limb pain, swelling, superficial venous engorgement and cords that can be touched along the vein. Positive results may be diseases: fulminant hepatic failure, hereditary prothrombin deficiency precautions Inappropriate crowd: pregnant women, newborns. Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process 1. The collected plasmas are numbered 1 to 5, and each is divided into 2 parts, one of which is subjected to isoelectric precipitation to extract prothrombin, and the other is extracted by cesium citrate adsorption method. 2. Isoelectric point precipitation method: the plasma is diluted 10 times with distilled water, the pH is adjusted to 5.3 with 5% glacial acetic acid, left at 4 ° C overnight, centrifuged at 3000 r / min for 15 min, the supernatant is discarded, and the precipitate is dissolved with physiological saline. spare. 3. Adsorption method of bismuth citrate: solution A was added to the plasma at a volume ratio of 10:1, stirred at 4 ° C for 30 min, allowed to stand for 30 min, centrifuged at 3000 r/min for 30 min, and the supernatant was discarded. The precipitate was washed with solution B, centrifuged at 3000 r/min for 15 min, and the supernatant was discarded. Repeat the wash 3 times. The precipitate was desorbed with solution C, stirred at 4 ° C for 0.5 to 1 h, and centrifuged at 3000 r / min for 15 min to take the supernatant. The supernatant was dialyzed against Solution D overnight at 4 °C. The supernatant was centrifuged at 3000 r/min for 15 min, which was a prothrombin solution. Not suitable for the crowd 1. Patients taking drugs such as oxidative drugs and steroid hormones may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. 3. Pregnant women and newborns are not suitable for this examination. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.