Plasma Thrombin Activity Assay

Plasma thrombomodulin activity assay is a measure of the activity of plasma thrombomodulin in humans, and whether the diagnosis is missing. Thrombin regulatory protein, also known as thrombomodulin, is an endothelial cell surface molecule that maintains intact blood vessel membranes and is one of the thrombin receptors expressed by vascular endothelial cells. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value 16 μg / L. Clinical significance Abnormal result The level of activity was less than 16 μg/L, suggesting that plasma thrombomodulin activity is too low. May have malignant blood diseases such as systemic lupus erythematosus, diabetes, glomerular disease, disseminated intravascular coagulation and leukemia, which require further examination. The people who need to be examined: weak, pale, and have a sickness. Loss of appetite and weight loss. Easy to bruise or hemorrhage, sore throat, bronchitis with headache, low fever, mouth pain and rash. Lymph nodes, especially in the throat, underarms, and groin. A person who feels uncomfortable under the left flank. Low results may be diseases: leukemia, lupus erythematosus precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process The thrombin standard was taken, and a standard solution having a titer of U, 5, 7, 8, 9, and 10 was prepared by using physiological saline. Take another 6×10cm plastic test tube, add 450μl of 0.1% fibrinogen solution, preheat for 5min in 37°C water bath, and then take 50μl of each of the above six concentrations of standard solution preheated at 37°C. Add to each of the above test tubes, immediately time and shake well, and record the coagulation time. Each concentration was measured 2 times, averaged, and a standard curve was made. The sample to be tested is diluted with physiological saline to a suitable concentration, 50 μl is taken, and measured in parallel according to the measurement method of the standard curve twice, averaged, and the titer of the sample is calculated according to a standard curve or a regression equation. Not suitable for the crowd 1. Patients taking drugs such as oxidative drugs and steroid hormones may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.