Plasma prokallikrein assay

Plasma kallikrein assay is a measure of plasma kallikrein in human plasma for thrombus and hemostasis screening. A kininogenase is a protease obtained by extracting and purifying mammalian pancreas, subchondral gland, and urine. It is widely found in urine, plasma and glandular tissue and promotes the release of active kinins from inactive kininogens. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The chromogenic substrate method was 98.03% ± 14.31%. Clinical significance (1) Elevation: pregnancy-induced hypertension syndrome, essential hypertension, cerebral infarction, cancer chemotherapy, hypercoagulable state, thrombotic disease. (2) reduction: congenital or acquired kallikrein deficiency (liver disease, renal hypertension, acute renal failure, infectious disease, cancer before chemotherapy, disseminated intravascular coagulation). The people who need to be examined have blood bubbles, subcutaneous hematoma, hemorrhage at the blood collection site, surgical wound bleeding, traumatic bleeding and visceral bleeding. Low results may be diseases: disseminated intravascular coagulation, cerebral infarction, renal hypertension, high results may be diseases: neonatal disseminated intravascular coagulation, thrombosis considerations Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd 1. Patients taking drugs such as oxidative drugs and steroid hormones may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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