Cholesterol ester to total cholesterol ratio

Cholesterol lipids also rise when cholesterol levels rise with age or dietary conditions. Because pure cholesterol is different from serum cholesterol matrix, the reaction rate is different, and the results of pure cholesterol standard in the enzymatic determination are often low. Therefore, it is recommended to use the standard reference serum as the standard to achieve the same reaction as the serum sample, and the standard reference serum application reference method is based on the standard cholesterol adhering to the international standard. The standard serum can be stored at -20 ° C for 1 year. It is not advisable to freeze and freeze repeatedly. Basic Information Specialist classification: cardiovascular examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Tips: This check needs an empty stomach. Normal value 0.6 to 0.8:1. Clinical significance (1) The ratio of obstructive jaundice in the jaundice of the liver is normal, and the ratio of hepatic jaundice is decreased. (2) Increased hepatocyte damage, early viral hepatitis, and familial lecithin cholesterol acyltransferase deficiency. High results may be diseases: hepatitis B precautions (1) When using an automatic analyzer, the reaction procedure can be designed according to the instrument conditions and reagent reaction conditions. Automated analyzers require fast enzyme reactions (preferably within 5 minutes). Reactive reagents can only be used for manual manipulation and colorimetrically until the reaction reaches the end. Reagents and specimens can be scaled up or down according to the cuvette volume. (2) In order to ensure that the enzyme reaction is rapid and completely beneficial to the long-term stable storage of the enzyme reagent, the enzyme preparation is required to have good performance and excess enzyme. After the enzyme reagent is formulated, it should be stored at 4 ° C for more than 3 days. Different batches of reagents should be checked for reaction arrival time, color stability and linear range. (3) If the cholesterol content in the sample exceeds the linear range of the reagent (the range of the general enzyme reagent should be within 5g/L), the specimen can be diluted with physiological saline and retested. The result must be multiplied by the dilution factor. (4) Serum bilirubin below 0.1g/L, ascorbic acid below 0.05g/L and mild hemolysis does not affect the results. The amount of other steroids in the serum is very small and does not cause significant interference. (5) When the instrument is stable and the operator is skilled, the CV within the batch is <1%, and the CV between the batches is <2%. (6) Because pure cholesterol is different from serum cholesterol matrix, the reaction rate is different, and the results of pure cholesterol standard in the enzymatic determination are often low. Therefore, it is recommended to use the standard reference serum as the standard to achieve the same reaction as the serum sample, and the standard reference serum application reference method is based on the standard cholesterol adhering to the international standard. The standard serum can be stored at -20 ° C for 1 year. It is not advisable to freeze and freeze repeatedly. Inspection process (1) Pipetting serum samples, standards, and water (blank) each at 20 μl. (2) Add 2 ml of enzyme reagent and mix for several seconds with a vortex mixer. (3) Insulation at 37 ° C for 10 to 15 minutes. (4) Colorimetric. Zero the reagent blank. The absorbance values ​​of the standard and serum samples were read separately. Not suitable for the crowd Generally there are no special taboos. Adverse reactions and risks It generally does not cause complications and harm.