Fetal hemoglobin (HbF) acid elution assay

The fetal hemoglobin (HbF) acid elution test is a test to test the Hbf content and distribution in fetal red blood cells, and is clinically proven for the determination of fetal thalassemia and aplastic anemia. HbF is different from other hemoglobin in that it is more resistant to acids than alkali. After the blood tablets were incubated in the acidic buffer, the other hemoglobin was eluted and stained pale, while the HbF was not eluted and stained red. A certain amount of blood is taken from the fetus. After the treatment, the blood sample is incubated in an acidic buffer, and other hemoglobin is eluted and dyed into pale, and HbF is not eluted and dyed red, thereby judging. Basic Information Specialist classification: maternity check check classification: blood test Applicable gender: whether women are fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally should be normal. Positive: Common in diseases such as heavy beta marine anemia. Tips: Actively cooperate with the doctor's work. Normal value Fetal hemoglobin (HbF) acid elution test was negative. Clinical significance Abnormal result Blood samples of heavy beta marine anemia, almost all red blood cells are stained red. Light beta marine anemia is rarely stained with red blood cells, and the staining is different. All cells of the hereditary fetal hemoglobin persistent syndrome were uniformly red, and the homozygous erythrocytes stained deeply and uniformly, while the heterozygous type stained slightly lighter, but also more uniform, and the erythrocyte staining with light beta marine anemia was shallow. Not different, so you can do the basis for differential diagnosis. A few HbF-containing red blood cells can also occur in certain cases such as aplastic anemia and hemolytic depletion. The population to be examined has pregnant women with thalassemia and other anemia. Positive results may be diseases: congenital aplastic anemia, pediatric globin dysplasia, thalassemia considerations Taboo before the examination: poor rest, improper diet, excessive fatigue. Requirements for inspection: Actively cooperate with the doctor's work. Inspection process A certain amount of blood is taken from the fetus. After the treatment, the blood sample is incubated in an acidic buffer, and other hemoglobin is eluted and dyed into pale, and HbF is not eluted and dyed red, thereby judging. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.