Ristomycin cofactor activity assay

The ristocetin cofactor activity assay utilizes the agglutination of normal platelets in the presence of ristocetin cofactor. The intensity of agglutination is related to the content and structure of vWF in the plasma to be tested. A standard curve was drawn based on the dilution of normal plasma and its corresponding agglutination reaction. Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The vWF ristocetin cofactor activity is 50% to 150%. Clinical significance Abnormal result The reduction of vWF is mainly used for the diagnosis and classification of von Willebrand disease. vWF increased vWF is an acute corresponding protein, which is increased in many cases, common in thrombotic diseases, such as myocardial infarction, glomerular disease, DM, pregnancy-induced hypertension and major surgery. The elderly population in the population to be examined, with limb pain, swelling, superficial venous engorgement and cords that can be touched along the vein. Precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. It is forbidden to take related drugs such as aspirin. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process The EDTA-Ha solution was swabbed, and the platelets were washed 4 times with ris-EDTA buffer, and then changed to anticoagulation with 0.73=01/L trisodium citrate solution, and washed with salt water and added with “rinsing method, ie light Lightly add the original plasma volume of citric acid brine along the wall for 4 weeks, do not suspend the blood and pull the plate, discard the net and cover it, repeat 1 shape. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.