Hemolysis test

The hemolysis test is a method for detecting red blood cell rupture and dissolution of red blood cells in a human body. Mainly used for the etiological diagnosis of hemolytic anemia. Hemolysis refers to the phenomenon of red blood cell rupture and dissolution. Many physical and chemical factors can cause hemolysis. In vitro, such as hypotonic solution, mechanical strong oscillation, sudden low temperature freezing (-20 ° C ~ -25 ° C) or sudden freezing, peracid or over-alkali, and alcohol, ether, soap, choline salt, etc. can cause Hemolysis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value The hemolysis rate of the glucose-free tube was less than 4.5% at 24 hours, and the hemolysis rate was less than 0.4% with glucose or ATP tube. Clinical significance Abnormal result Hemolysis abnormality 1, hereditary spherocytosis is significantly increased, and can be corrected with glucose and ATP. 2, hereditary non-spherical erythrocyte hemolytic anemia can also be increased, and can be corrected by glucose or ATP, respectively. 3, acid kinase deficiency, autoimmune hemolytic anemia, paroxysmal nocturnal hemoglobinuria, drug-induced hemolysis, etc., plus glucose can not be corrected, plus ATP can be corrected. The people who need to be examined have weak, weak, sleepy, pale skin, mucous membranes, palpitations, dizziness, headache, tinnitus, vertigo, inattention, and lethargy. Precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. The spleen cells before the test must be placed in an ice bath to maintain their vitality. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. During the test, if the weather is cold, the test tubes and slides used should be preheated to prevent the agarose from solidifying. It is necessary to keep the temperature at 45 °C during operation. After mixing the droplets on the slide, quickly cover the slides to prevent the agar from spreading out due to solidification and affecting the counting. Inspection process Take 3 tubes, add 2.0 ml of freshly prepared 2% rabbit blood physiological saline suspension, add 2.0 ml of normal saline as control tube, and add 2.0 ml of test solution dilution (seed solution). The third tube was added with a test solution dilution solution (diethyl ether) 2.0 ml, shaken, and allowed to stand at 37 ° C for 1 hour. Not suitable for the crowd People with a significant tendency to bleed. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.