Polyamine test

The condensed amine test is a test for the detection of blood using a condensed amine technique for the diagnosis of cross-matching between two pairs of blood types that are stable in coexistence to prevent or diagnose neonatal hemolytic disease caused by ABO blood group incompatibility. The principle is to use low-ion solution to reduce ionic strength, accelerate molecular motion, reduce red cell electrostatic repulsion, shorten red blood cell spacing, promote non-specific agglutination of antibody binding in erythrocyte antigen and serum (plasma), and non-specific agglutination after additional false agglutination Dispersion immediately, and specific aggregation caused by antigen-antibody reactions will still be present. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Indicates blood type matching, which can be transfused. Positive: It is suggested that there is no pairing between the two blood types, and blood transfusion cannot be performed. Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Normal value The clot was dispersed within 1 minute and the test result was negative. Clinical significance The test results were positive, that is, the clot did not disperse within 1 minute, and the two blood types were diagnosed to be unpaired, and blood transfusion could not be performed. People who need to be examined need people who are transfused. Positive result may be disease: hemolytic jaundice precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process The recipient red blood cells were mixed with physiological saline to prepare a 3-5% cell suspension. The amount of the sample was 2 drops of serum and 1 drop of red blood cell suspension. Supervisor: blood donor serum + donor red blood cell suspension; secondary tube: recipient red blood cell suspension + donor serum; (yin and yang control tube set). Each tube was mixed with 0.6 ml of a low ionic medium and allowed to stand at room temperature for 1 minute. Add 2 drops of the condensed amine solution, mix and set at room temperature for 15 seconds. 1000 g (3000 rpm), centrifuge for 18 seconds, discard the supernatant, leave about 2 drops of liquid at the bottom of the tube, and shake gently. Test tubes to see if a clot forms. If no clots are formed, redo the previous test. Add 2 drops of the suspension, mix gently, and observe the results under the naked eye or under the microscope. Not suitable for the crowd No taboos. Adverse reactions and risks No complications.