Immunoelectrophoresis

Immunoelectrophoresis is a qualitative method used to analyze the composition of antigens by combining agarose electrophoresis with two-way agar diffusion. Founded in 1953 by Grabar and Williams. Due to the high specificity of antigen-antibody reaction and the rapid, sensitive and high-resolution electrophoresis separation technology, this technology is an immunological basic technology widely used in biomedical field. High sex and strong resolution. However, the substance to be analyzed must be antigenic, and the antiserum must contain all of the antibody components. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally it is normal. Positive: Common in multiple myeloma, liver disease, systemic lupus erythematosus and other diseases. Tips: Taboo overeating, especially spicy food. Normal value The test result was negative. Clinical significance Abnormal result In recent years, this method is mainly used for the analysis of serum protein components, such as multiple myeloma, liver disease, systemic lupus erythematosus, etc.; detection of the purity of antigens and antibodies; research on the components of antibodies. It is also commonly used to detect hepatitis B surface antigen (HBsAg), alpha-fetoprotein, and qualitative and semi-quantitative immunoglobulins in serum. People who need to be checked Those with related diseases and symptoms, especially those with liver disease, and newborns can be tested for prevention. Positive results may be diseases: herpes simplex keratoconjunctivitis, Staphylococcus aureus meningitis, pediatric trematode considerations Taboo before the test: taboo overeating, especially spicy food. Taboo strenuous exercise. Requirements for examination: fasting blood test. Follow the doctor's request. Inspection process The antigen is first added to the pores of the agar plate for electrophoresis, and then a transverse groove is dug in the center of the agar plate, and the corresponding corresponding immune serum is added. After the two are mutually diffused for a certain period of time, the antigen and antibody ratios are optimal. A precipitation arc is formed at the place. According to the number, position and shape of the precipitation arc, the components contained in the sample can be analyzed by referring to the electropherogram formed by the known antigen and the antibody. This method has a small amount of sample, high specificity and strong resolution. However, the substance to be analyzed must be antigenic, and the antiserum must contain all of the antibody components. Not suitable for the crowd No taboos. Adverse reactions and risks No complications.