simple thrombin generation test

Because of the shortcomings of Bigg's thromboplastin test (TGT), such as labor, blood consumption and time-consuming, Xu Fuyan et al first reported the simple thromboplastin test (STGT) and its corrective test in 1965. It is an endogenous coagulation pathway, especially a diagnostic test for the diagnosis of hemophilia. The trial uses the subject to dilute whole blood as a source of all coagulation factors; it replaces PF3 with its erythrocyte lysate, and thus it is also an endogenous coagulation pathway. Screening test. More than 15 s is poor production of thromboplastin. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Reminder: Stop using anticoagulant drugs and antiplatelet drugs before the test, because these drugs can prolong the test results. Normal value 10 to 14 s, more than 15 s is abnormal. Clinical significance Abnormal result 1. The lack of factor VIII is found in hemophilia A, von Willebrand disease (VWD), DIC, etc. 2, the lack of factor IX found in hemophilia B, liver disease, vitamin K deficiency, oral anticoagulant, DIC and so on. 3. The lack of factor XI is found in factor XI deficiency, liver disease, DIC, and the like. 4. Anticoagulant substances in the blood are found in anti-factor VIII antibodies. People who need to be tested Abnormal liver function, hemophilia, hemophilia B, liver disease, vitamin K deficiency. High results may be diseases: diffuse intravascular coagulation, hemophilia in children, hemophilia precautions Before the test: Stop the application of anticoagulant drugs and antiplatelet drugs, because these drugs can prolong the test results. During the examination: should relax, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inspection process 1. Take 0.4ml of whole blood of the subject, add 5ml of water, mix and dissolve into dissolved blood. 2, aspirate 0.5ml of dissolved blood, add 0.308mol / L sodium chloride solution 0.5ml, mix, so that isotonic sodium chloride hemolysis, plasma dilution is about 1:50. 3. Set in a 37 ° C water bath for 2 min. 4, suck 0.025mol / L calcium chloride solution 0.25ml (pre-warmed to 37 ° C) into the upper liquid, start the stopwatch, then insert a bamboo stick in the test tube, and when the filament is formed, pick it up (this is incubation) Mixed solution). 5. Add 0.1 ml of 0.025 mol/L sodium chloride solution to 6 identical small test tubes and place in a 37 ° C water bath. 6. At 1min45s, 0.1ml of the incubation mixture was injected into the first tube containing the calcium chloride solution. In the second 2min, 0.1ml of normal matrix plasma (pre-warmed to 37 °C) was added to the same tube. Start the second stopwatch and record the solidification time. 7. Repeat step 2 of “(6)” for a total of 6 times. The shortest setting time was used as the most valuable reading in this test. Not suitable for the crowd 1. Patients who have taken thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, dizziness or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 2. Risk of infection: If you use an unclean needle, you may be at risk of infection.