Single-phase agar diffusion test

The one-way agar diffusion test is a commonly used method for quantitative detection of antigens. The appropriate amount of antibody is mixed with the agar, cast into a plate, and after solidification, the plate is perforated, and the antigen is added to the well, and the antigen is diffused around the well, and is bound to the antibody in the agar while diffusing. After a certain period of time, a white precipitate ring was formed at a suitable ratio between the two. The diameter of the precipitation ring is proportional to the concentration of the antigen. If a standard curve is prepared with different concentrations of standard antigen in advance, the antigen content in the sample can be determined from the curve. This test is mainly used to detect the content of various immunoglobulins in serum and various complement components in serum, and has high sensitivity. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: It is generally normal. Positive: Common in immune complex disease, acute nephritis, hepatitis and other diseases. Tips: Taboo overeating and strenuous exercise. Normal value The test result was negative. Clinical significance Abnormal result The content of C3 can be detected by single-phase immunodiffusion method, and the change of its content can help the diagnosis and prognosis of certain diseases, such as immune complex disease, acute nephritis and hepatitis. People who need to be checked It can detect the levels of IgG, IgA and IgM in the serum of normal people or patients. Both the elderly and the newborn need to be examined. Positive results may be diseases: hepatitis, acute nephritis syndrome, acute nephritis precautions Taboo before the test: taboo overeating and strenuous exercise. Especially diet-stimulating foods such as wine. Requirements for examination: fasting blood test. Inspection process A well plate containing a specific antibody is perforated, and a quantitative antigen is added to the well. When the antigen is diffused to the periphery and combined with the antibody in the agar, a white precipitate ring is formed, and the diameter or area thereof is positively correlated with the antigen concentration. At the same time, standard curve or standard reference protein can be used to make the standard curve, which can be used to quantitatively detect the antigen concentration (mg/ml or U/ml) of the unknown specimen. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.