IgG anti-B-titer assay

The IgG anti-B-potency assay is to check the presence or absence of IgG-like anti-B antibodies in the serum of pregnant women and titrate their titers to assess the likelihood of ABO-HDN development. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether women are fasting: fasting Tips: On an empty stomach, taking blood in the morning is better. Normal value IgG anti-B titer <1:64. Clinical significance Abnormal results: IgG anti-B titer ≥ 1:64, children may develop ABO-HDN, manifested as fetal edema and stillbirth, such as abortion, premature delivery, stillbirth or death within a few hours after birth; children with severe anemia, jaundice, nuclear jaundice And other symptoms. People who need to be checked A couple of pregnant women with different blood types or different maternal and child blood types. Precautions Before the examination: fasting, taking blood in the morning is better. When checking: Relax your body and actively cooperate with your doctor. Inspection process Collect venous blood of the test subject, take 50 μl of serum sample and add an equal volume of bovine serum albumin, incubate for 30-60 minutes in a 37-degree water bath, and then dilute the diluted serum and add the labeled serum to the main gel. In the globulin card, an equal amount of B-type 0.5% red blood cell suspension was added to all wells and incubated at 37 degrees for 5 minutes, centrifuged for 5 minutes in a dedicated centrifuge, and the results were observed. Not suitable for the crowd 1. Patients who have taken thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.