autohemolysis test

Normal human blood is incubated at 37 ° C for 24 to 48 hours, will gradually produce mild hemolysis, but hereditary spherocytosis and hereditary non-spherical erythrocyte hemolytic anemia, etc., self-hemolysis can be significantly enhanced. After adding glucose or ATP, or to obtain different degrees of correction. Therefore, it can be used for the differential diagnosis of certain hemolytic anemia. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally, the group is in a normal state. Positive: Found in hemolytic anemia. Tips: Actively cooperate with the doctor during the examination. Normal value Qualitative test: negative. Quantitative test: 24h No sugar: 0.0005 ~ 0.005; hemolysis: (0.05% ~ 0.5%). Sugar added: 0 ~ 0.004; hemolysis: (0 ~ 0.4%). 48h No sugar: 0.004 ~ 0.045; hemolysis: (0.4% ~ 4.5%). Sugar added: 0.0003 ~ 0.006; hemolysis: (0.03% ~ 0.6%). Clinical significance Patients with PNH are positive or have an increased hemolysis rate and can be used as a screening test for PNH. Some of the autoimmune anemia can be positive, and false positives can occur in leukemia and bone marrow sclerosis. Positive results may be diseases: hemolytic anemia, hemolytic anemia in children, drug-related immune hemolytic anemia considerations 1. The lysed blood vessel is diluted 200 times, and the measuring tube is diluted 10 times, so multiply by 20. 2. If the hemolysis is more obvious, the dilution factor can be increased. 3. If there is no photoelectric colorimeter, only the qualitative report is used, that is, the hemolysis is observed with the naked eye. 4, according to needs, only 24h incubation, can also be used as a reference. 5. The operation process should be strictly sterile. Inspection process The red blood cells were placed in a sucrose solution with a low ion concentration, and at 37 ° C, the binding of complement and red blood cells was promoted, and whether the red blood cells were destroyed or not hemolysis was observed. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.