Sucrose hemolysis test

The sucrose hemolysis test means that the red blood cells are placed in a sucrose solution having a low ion concentration, and at 37 ° C, the binding of complement and red blood cells can be promoted, and whether the red blood cells are destroyed or hemolysis is observed. This test is often used in conjunction with the acid dissolution test as a simple screening test for arrayed sleep hemoglobinuria. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Paroxysmal nocturnal hemoglobinuria and other diseases. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Qualitative negative The quantitative hemolysis rate is less than 5%. Clinical significance 1, paroxysmal nocturnal hemoglobinuria is positive, hemolysis rate > 10%. 2, the test is highly sensitive, a small number of aplastic anemia, cellular anemia, hereditary spherocytosis and autoimmune hemolytic anemia can also be positive. Therefore, this test is a diagnostic screening test for paroxysmal nocturnal hemoglobinuria, the specificity is not high, and the positive results need to be tested for acid hemolysis. 3, some granulocyte leukemia, bone marrow fibrosis and some anemia of some red blood cells may also be weakly positive, but hereditary erythrocytosis syndrome (type 2) is negative. Positive results may be diseases: leukemia, hemolytic anemia in children First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should fast. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The red blood cells were placed in a sucrose solution with a low ion concentration, and at 37 ° C, the binding of complement and red blood cells was promoted, and whether the red blood cells were destroyed or not hemolysis was observed. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.