Pancreatic antigen

Pancreaticon cotetal antigen (POA) was first discovered in Banwo in 1974. In recent years, serum MAA was determined by monoclonal antibody to establish IRMA method. The antigen of the pancreatic embryo is raised by the fetal pancreas. POA is present in serum in the form of a complex having a molecular weight of 9000 kD, but can still be degraded into a component having a molecular weight of 40 kD. It is not accompanied by any known plasma protein carrier, which is not affected by DNase and RNase, but can be hydrolyzed by trypsin, papain or pepsin. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: According to the nutritional status of the whole body, milk, eggs, fruits, soy milk, etc. are given in the meal. Normal value Serum <2.0 kU/L. Clinical significance The increase in serum POA was found in 77.7% of patients with pancreatic cancer, 70% for gallbladder cancer, cholangiocarcinoma, 57.1% for colorectal cancer, and 28.0% for gastric cancer, but the positive rate of benign pancreatic disease accounted for 40.0%. In 1980, HObbs detected POA in serum of 288 different malignant tumors by immunoelectrophoresis. The positive rate of pancreatic cancer is 95%. Combined with CA19-9, CA242 can improve specificity. High results may be diseases: cholangiocarcinoma, gallbladder cancer, pancreatic cancer The specificity of pancreatic embryo antigen is not high, but it can be used to observe the efficacy of pancreatic cancer resection, an indicator of pancreatic cancer recurrence monitoring. Inspection process Operate in strict accordance with the instructions. The basic procedure is to first wash the microplate with the anti-shhinocerebral antigen antibody, and then add the test specimen, the negative and positive reference serum, and after the heat preservation and washing, add the enzyme labeled anti-shhin embryo antigen antibody, and wash the same method. , add substrate color. For quantitative measurement, 100 μl of a substrate solution prepared by adding o-phenylenediamine and hydrogen peroxide per well was placed in a water bath at 37 ° C for 15 min to terminate the reaction, and the results were observed. For qualitative determination, it is best to use a substrate solution prepared with tetramethylbenzidine (TMB) and hydrogen peroxide, 100 μl per well, and placed in a 37 ° C water bath for 5 to 15 minutes. As a result, the A492nm value was read with an enzyme standard colorimeter. Quantitative determination can refer to the concentration of pancreatic embryo antigen in the standard solution as the abscissa, to determine the value of the hole A as the ordinate, draw a standard curve on the semi-logarithmic coordinate paper, and find the pancreas from the table according to the A value of the sample to be examined. Antigen content. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.