Platelet Aggregation Test (PAgT)

The adhesion between platelets and platelets is called platelet aggregation, which is an important function of platelet thrombosis and an important hemostatic function of platelets. The platelet aggregation test mainly reflects the aggregation function of platelets. Elevation is seen in myocardial infarction, arteriosclerosis, cerebrovascular disease, diabetes, and the like. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: If you take this kind of medicine, you should stop taking it 1 week before the test. Oral contraceptives, late pregnancy, high-fat diets, and smoking can increase platelet aggregation. Normal value Large agglomerated particles appeared within 10 to 15 seconds. Clinical significance Reduced in platelet weakness, giant platelet syndrome, primary thrombocytopenic purpura, macroglobulinemia, acute white blood, aplastic anemia. Elevation is seen in myocardial infarction, arteriosclerosis, cerebrovascular disease, diabetes, and the like. Precautions 1. Application of antiplatelet drugs can reduce platelet aggregation. If you take these drugs, you should stop taking them 1 week before the test. 2, oral contraceptives, late pregnancy, high-fat diet and smoking, etc., can increase platelet aggregation. Inspection process (1) 4.5 ml of blood was smoothly taken from the elbow vein with a silicified or plastic syringe, and a siliconized centrifuge tube containing 0.129 mol/L sodium citrate 0.5 ml was injected. (2) Centrifuge at 1000 r/min for 10 min, carefully remove the upper plasma (PRP), count the platelets and adjust to 250×109/L. (3) Centrifuge the remaining blood at 3000r/min for 20min. The relatively transparent liquid in the upper layer is PPP, and the platelets are adjusted to (10-20)×109/L. (4) Put the PRP specimen into the measuring well to adjust the transmittance to 10%, and add a stirring magnetic rod to preheat for 3 min at 37 °C. (5) An inducer was added to the PRP, and stirring was started (1000 r/min), and the aggregation waveform was recorded. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.