platelet adhesion function test

Under physiological conditions, platelets have a function of adhering to the surface of foreign bodies called platelet adhesion. The method for determining platelet adhesion is called the platelet adhesion test (PAdT). PAdT has two types, in vivo and in vitro. The former is not commonly used, and the latter is simple and versatile. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Common in vascular hemophilia. Normal value: Glass ball method: 52.6-71.4% Glass bead method male: 28.9-40.9% Glass bead column method female: 34.2-44.6% Above normal: Common in hypercoagulable states and thrombotic diseases. negative: Positive: Tips: Blood must be taken smoothly, blood should not be mixed with vacuoles and clots. Normal value (1) Glass ball method: 52.6% to 71.4%. (2) Glass bead column method, male: 28.9% to 40.9%; female: 34.2% to 44.6%. Clinical significance (1) Platelet adhesion rate reduces vascular hemophilia (VWD), giant platelet syndrome, Ai-Tang syndrome and cirrhosis, uremia, myeloproliferative syndrome, taking platelet-suppressing drugs. (2) Increased platelet adhesion rate Hypercoagulable state and thrombotic diseases, such as myocardial infarction, angina pectoris, cerebrovascular disease, diabetes, deep vein thrombosis, pregnancy-induced hypertension syndrome, glomerular disease, etc. Low results may be diseases: vascular hemophilia in children, thrombocytopenic purpura high results may be diseases: children with essential thrombocytopenia precautions (1) Blood collection must be smooth, and blood should not be mixed with vacuoles and clots. (2) The size of the foreign body surface is proportional to the value of platelet adhesion. (3) The contact time has a significant effect on platelet adhesion, and the contact time is short, and the platelet adhesion rate is low. Inspection process (1) Glass ball method: 1 Take 4.5ml of venous blood, place it in a centrifuge tube containing 0.129mol/L sodium citrate solution 0.5ml, and mix gently. 2 Immediately take 1.5ml of blood sample with a micro-injector, place it in a spherical bottle, place the spherical bottle on the rotating device, and rotate it at a speed of 3r/min for 15min to make the blood fully contact with the bottle wall. 3 Then use the two micro-most syringes to accurately absorb 1.0ml of blood from the centrifuge tube (before contact) and the spherical bottle (after contact), respectively, in two large test tubes, and then add 0.109mol/L each. 19 ml of sodium acetate solution, covered with a plastic film, repeatedly poured 3 times, mixed, and placed at room temperature for 2 h. 4 Take the supernatant to accurately count the platelets. (2) Glass bead column method: 1 Connect the ends of the bead column to the needle and syringe, respectively. 2 lines of elbow vein puncture. 3 When the blood contacts the bead column, immediately start the stopwatch and master the speed of the blood passing through the bead column. In the four-part bead column, the blood passes through each segment for 5s for 20s. 4 Then pump for another 6 to 7 seconds at the same speed, then pull out the needle. 5 Collect the blood in the plastic tube before and after the glass bead column, and make platelet counts respectively. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.