Platelet granule membrane protein 140 assay

Platelet Granule Membrane Protein 140 Assay (GMP-140) is an abnormality in the determination of platelet cell adhesion receptors. GMP-140 is rapidly stimulated by thrombin, histamine, complement C5b9, and α-granules and rod-shaped small body membranes. It is expressed on the cell membrane by fusion with the cell membrane, mediates the initial adhesion of leukocytes and endothelial cells, mediates the binding of platelets to various white blood cells, and plays an important role in inflammation and thrombosis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: The number of platelets is greater than 6.3%, which is common in diseases such as hypertension. Tips: Do not eat too greasy, high-protein foods the day before, and avoid heavy drinking. Normal value Normal human GMP-140 is positive, and the number of platelets is 0-6.3%. Clinical significance Abnormal results: the test results showed positive, that is, GMP-140 is greater than 10ug/L, and the number of positive platelets is greater than 6.3%. In patients with coronary heart disease, hypertension, cerebral arteriosclerosis, hyperlipoproteinemia, diabetes, etc. and prethrombotic state The 100% separation of GMP-140 positive platelets increased to varying degrees. People who need to be examined: people with diabetes, people with high blood pressure and symptoms of coronary heart disease. Positive results may be diseases: coronary heart disease considerations Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.