Platelet electrophoresis time measurement

The internal viscosity of platelets is greater than that of red blood cells. An increase in the number of platelets, an increase in adhesion and aggregation, and an increase in emissions all increase blood viscosity. The factors affecting platelet aggregation are the same as those affecting red blood cells. However, in low rheological conditions, platelets are more likely to aggregate. Platelet adhesion, aggregation, blood viscosity, plasma composition and HCT increased, platelet deformability decreased, blood flow velocity slowed and abnormal flow, and eddy currents, is a favorable condition for thrombosis. Damage to the blood vessel wall causes platelet adhesion, aggregation, and changes in platelet number and morphology, resulting in decreased blood flow. The blood flow slows down or flows through the blood vessels to bend, bifurcate, narrow, or the blood vessel wall is roughened by pathological damage. The platelets tend to adhere to the wall of the tube, accumulate and release procoagulant substances, which make blood coagulation and thrombosis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: There are many factors affecting the speed of cell migration, so you should pay attention to control. Such as medium ionic strength, electric field strength and humidity. Normal value The normal range of inspection is: 16.4 to 23.2 s. Clinical significance Time prolongs ischemic stroke, myocardial infarction, angina pectoris, coronary atherosclerotic heart disease (coronary heart disease), occlusive thromboangiitis (vasculitis), pulmonary heart disease, hypertension, chronic bronchitis. High results may be diseases: vascular inflammation considerations There are many factors affecting the speed of cell migration, and attention should be paid to control. Such as medium ionic strength, electric field strength and humidity. Inspection process (1) The prepared diluted cell suspension is placed in a square glass tube, and then the agar tube is placed on both ends, placed on the electrophoresis tube rack, placed on a microscope stage, and inserted into the electrode. (2) Turn on the electrophoresis: use the reverse switch to change the polarity of the two electrodes, use the micrometer to measure the time required for the cell to move a certain distance (s) (-t), and record 20 cells in the direction of both ends. The average value of the moving time (-t) is calculated as the electrophoresis speed (v), V = s / -t. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.