Platelet electrophoresis time measurement

The time required for platelets to move a certain distance under the action of a DC electric field is called platelet electrophoresis time. The factors affecting the electrophoresis time are mainly related to the increase of plasma lipids, globulin and fibrinogen and the increase of plasma viscosity. Time prolongs ischemic stroke, myocardial infarction, angina pectoris, coronary heart disease, vasculitis, pulmonary heart disease, hypertension, chronic bronchitis. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: There are many factors affecting the speed of cell migration, so you should pay attention to control. Such as medium ionic strength, electric field strength and humidity. Normal value Cell electrophoresis electrophoresis method 16.4 ~ 23.2 s. Clinical significance Time prolongation: ischemic stroke, myocardial infarction, angina pectoris, coronary heart disease, vasculitis, pulmonary heart disease, hypertension, chronic bronchitis. The platelet electrophoresis of patients with coronary heart disease is parallel with the degree of myocardial ischemia. The thrombosis-based disease is positively correlated with the erythrocyte electrophoresis time. The electrophoresis rate of cancer cells is higher than that of normal cells, and the higher the degree of canceration, the higher the electrophoresis rate. . People who need to be examined: people with symptoms such as headache, dizziness, tinnitus, shortness of breath, insomnia, and numbness of limbs. High results may be diseases: vascular inflammation considerations There are many factors affecting the speed of cell migration, and attention should be paid to control. Such as medium ionic strength, electric field strength and humidity. Inspection process (1) The prepared diluted cell suspension is placed in a square glass tube, and then the agar tube is placed on both ends, placed on the electrophoresis tube rack, placed on a microscope stage, and inserted into the electrode. (2) Turn on the electrophoresis: use the reverse switch to change the polarity of the two electrodes, use the micrometer to measure the time required for the cell to move a certain distance (s) (-t), and record 20 cells in the direction of both ends. The average value of the moving time (-t) is calculated as the electrophoresis speed (v), V = s / -t. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.