thromboxane B2

Thromboxane B2 is the content of TXA2 estimated by the stable hydrolysis product TXB2 of thromboxane A2. For the detection of primary thrombocytopenic purpura, coronary heart disease, thrombotic disease. Thromboxane A2 (TXA2) is a metabolite of platelet arachidonic acid. Thromboxane A2 (TXA2) is a strong platelet aggregation activator, but its half-life is only 30 seconds. Therefore, the stable hydrolysis product TXB2 is detected to estimate TXA2. content. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Taking aspirin anti-inflammatory drugs can reduce the level of thromboxane, so it is forbidden to take this drug before the test. Normal value Male: (132 ± 55) ng / L. Female: (116 ± 30) ng / L. Clinical significance Changes in thromboxane levels are seen in: atherosclerosis, angina pectoris, coronary heart disease, diabetes, hyperlipidemia, etc. Increased TXA2/PGI2 ratio is prone to platelet aggregation, thrombosis, atherosclerosis and coronary heart disease. There was a significant increase in TXB2 in the plasma of hemorrhage, injury, and endotoxin shock, which was associated with increased lung circulation resistance during shock. In patients with chronic renal failure, TXB2 and 6-keto-PGF1α decreased in the urine. There were also significant changes in urinary PG in patients with renal vascular hypertension, nephrotic syndrome, and BATTER syndrome. TXA2 is altered in arterial tissue of patients with malignant tumors. When PGI2 and TXA2 are normal, it can prevent tumor cells from invading platelets and adhering to the surface of blood vessels. Factors that inhibit platelet TXA2 production and increase vascular endothelial cell PGI2 production have anti-tumor metastatic effects. People who need to be examined: people with symptoms of purpura on the skin's mucous membranes. Low results may be diseases: high results of chronic renal failure may be diseases: atherosclerosis, coronary heart disease considerations Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process (1) Sample extraction is the same as prostaglandin assay. (2) Take 8mm × 75mm glass tube, total counting tube (T) plus PBS300μl, non-specific binding tube (NSB) plus PBS200μl, zero standard tube (S0) plus PBS100μl, standard curve tube (S1 ~ 7) plus different concentration standards 100 μl of the working solution, and 100 μl of the purified sample solution was added to the measuring tube. (3) Add 100 μl of diluted antiserum to S0, S1 to 7 and the measuring tube, and let 4 °C for 6 to 12 hours. (4) Add 100 μl of [3H]-TXB2 working solution to all tubes, and let 4 °C for 6 to 12 hours. (5) In addition to the T tube, 100 μl of activated carbon separating agent was added, mixed and placed at 4 ° C for 15 min, and centrifuged at 3500 r / min (4 ° C) for 15 min. (6) The supernatant was all decanted into a counting bottle to which 11 ml of scintillation fluid was previously added, thoroughly mixed, and after 12 hours of standing, the radioactivity was measured on a liquid scintillation counter. (7) On the semi-logarithmic paper, LogitB/B0 is the ordinate, and each standard tube TXB2(pg) is the abscissa. The standard curve is drawn according to the linear regression method, and then the LogitB/B0 value of the sample tube is taken from the standard curve. Determine the sample tube concentration, such as the liquid scintillation counter with a data processing system, can directly give the standard curve, the relevant parameters and sample tube concentration. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.